Arl13b regulates endocytic recycling traffic

Duarte C. Barral, Salil Garg, Cristina Casalou, Gerald F M Watts, José L. Sandoval, José S. Ramalho, Victor W. Hsu, Michael B. Brenner

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)
6 Downloads (Pure)

Abstract

Intracellular recycling pathways play critical roles in internalizing membrane and fluid phase cargo and in balancing the inflow and outflow of membrane and cell surface molecules. To identify proteins involved in the regulation of endocytic recycling, we used an shRNA trafficking library and screened for changes in the surface expression of CD1a antigen-presenting molecules that follow an endocytic recycling route. We found that silencing of the ADP-ribosylation factor (Arf)-like small GTPase Arl13b led to a decrease in CD1a surface expression, diminished CD1a function, and delayed CD1a recycling, suggesting that Arl13b is involved in the regulation of endocytic recycling traffic. Arl13b appears to be required for the major route of endocytic trafficking, causing clustering of early endosomes and leading to the accumulation of endocytic cargo. Moreover, Arl13b colocalized with markers of the endocytic recycling pathway followed by CD1a, namely Arf6 and Rab22a. We also detected an interaction between Arl13b and the actin cytoskeleton. Arl13b was previously implicated in cilia formation and function. Our present results indicate a previously unidentified role for Arl13b in endocytic recycling traffic and suggest a link between Arl13b function and the actin cytoskeleton.

Original languageEnglish
Pages (from-to)21354-21359
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number52
DOIs
Publication statusPublished - 26 Dec 2012

Keywords

  • Ciliary traffic
  • Immune response
  • Lipid antigens
  • Vesicle traffic

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