Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration

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Abstract

The Arf-like protein Arl13b has been implicated in ciliogenesis and Sonic hedgehog signaling. Furthermore, we have previously shown that it regulates endocytic recycling traffic and interacts with actin. Herein, we report that the non-muscle myosin heavy chain IIA, also known as Myh9, is an Arl13b effector. Moreover, we found that both proteins localized to circular dorsal ruffles (CDRs) induced by platelet-derived growth factor stimulation and are required for their formation. CDRs are ring-shaped actin-dependent structures formed on the dorsal cell surface and are involved in diverse processes, such as macropinocytosis, integrin recycling, internalization of receptor tyrosine kinases and cell migration. We found that Arl13b or Myh9 silencing impaired cell migration, suggesting that Arl13b is required for this function through the interaction with Myh9. Moreover, Arl13b silencing impaired neural crest cell migration in zebrafish embryos. Furthermore, we showed that Arl13b is required for the formation of CDRs in migrating cells. Thus, our results indicate a new role for Arl13b in actin cytoskeleton remodeling through the interaction with Myh9, by driving the formation of CDRs necessary for cell migration.
Original languageEnglish
Pages (from-to)2709-2722
Number of pages13
JournalJournal Of Cell Science
Volume127
Issue number12
DOIs
Publication statusPublished - 15 Jun 2014

Fingerprint

Myosin Heavy Chains
Cell Movement
Actins
Hedgehogs
Neural Crest
Platelet-Derived Growth Factor
Recycling
Receptor Protein-Tyrosine Kinases
Zebrafish
Actin Cytoskeleton
Integrins
Embryonic Structures
Proteins

Keywords

  • Arl13b
  • Myosin heavy chain IIA
  • Myh9
  • Circular dorsal ruffle
  • Small G protein
  • Actin cytoskeleton
  • Platelet-derived growth factor signaling
  • Wound healing

Cite this

@article{52d27211cc37467daa97d34912b6762d,
title = "Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration",
abstract = "The Arf-like protein Arl13b has been implicated in ciliogenesis and Sonic hedgehog signaling. Furthermore, we have previously shown that it regulates endocytic recycling traffic and interacts with actin. Herein, we report that the non-muscle myosin heavy chain IIA, also known as Myh9, is an Arl13b effector. Moreover, we found that both proteins localized to circular dorsal ruffles (CDRs) induced by platelet-derived growth factor stimulation and are required for their formation. CDRs are ring-shaped actin-dependent structures formed on the dorsal cell surface and are involved in diverse processes, such as macropinocytosis, integrin recycling, internalization of receptor tyrosine kinases and cell migration. We found that Arl13b or Myh9 silencing impaired cell migration, suggesting that Arl13b is required for this function through the interaction with Myh9. Moreover, Arl13b silencing impaired neural crest cell migration in zebrafish embryos. Furthermore, we showed that Arl13b is required for the formation of CDRs in migrating cells. Thus, our results indicate a new role for Arl13b in actin cytoskeleton remodeling through the interaction with Myh9, by driving the formation of CDRs necessary for cell migration.",
keywords = "Actin cytoskeleton, LOCALIZATION, Arl13b, PROTEINS, Wound healing, MACROPINOCYTOSIS, Circular dorsal ruffle, Small G protein, ZEBRAFISH, Platelet-derived growth factor signaling, PRIMARY CILIA, Myosin heavy chain IIA, ACTIN, Myh9, LEAD, JOUBERT-SYNDROME, RECEPTOR, ROLES, Arl13b, Myosin heavy chain IIA, Myh9, Circular dorsal ruffle, Small G protein, Actin cytoskeleton, Platelet-derived growth factor signaling, Wound healing",
author = "Cristina Casalou and Cec{\'i}lia Seixas and Ana Portelinha and Petra Pintado and Mafalda Barros and Ramalho, {Jos{\'e} da Silva} and Lopes, {Susana Santos} and Duarte Barral",
note = "info:eu-repo/grantAgreement/FCT/SFRH/SFRH{\%}2FBPD{\%}2F32323{\%}2F2006/PT# info:eu-repo/grantAgreement/FCT/SFRH/SFRH{\%}2FBPD{\%}2F78561{\%}2F2011/PT# info:eu-repo/grantAgreement/FCT/SFRH/SFRH{\%}2FBD{\%}2F68502{\%}2F2010/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/103981/PT# This work was supported by a Marie Curie International Reintegration Grant [grant number Marie Curie_PIRG05-GA-2009-247726 to D.C.B.]; by postdoctoral fellowships from Fundacao para a Ciencia e Tecnologia (FCT) [grant numbers SFRH/BPD/78561/2011 to C.C., SFRH/BPD/32323/2006 to C.S.]; a PhD fellowship from FCT [grant number SFRH/BD/68502/2010 to A.P.]; and a FCT grant [grant number PTDC/SAU-OBD/103981/2008 to S.L.].",
year = "2014",
month = "6",
day = "15",
doi = "10.1242/jcs.143446",
language = "English",
volume = "127",
pages = "2709--2722",
journal = "Journal Of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "12",

}

TY - JOUR

T1 - Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration

AU - Casalou, Cristina

AU - Seixas, Cecília

AU - Portelinha, Ana

AU - Pintado, Petra

AU - Barros, Mafalda

AU - Ramalho, José da Silva

AU - Lopes, Susana Santos

AU - Barral, Duarte

N1 - info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F32323%2F2006/PT# info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F78561%2F2011/PT# info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F68502%2F2010/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/103981/PT# This work was supported by a Marie Curie International Reintegration Grant [grant number Marie Curie_PIRG05-GA-2009-247726 to D.C.B.]; by postdoctoral fellowships from Fundacao para a Ciencia e Tecnologia (FCT) [grant numbers SFRH/BPD/78561/2011 to C.C., SFRH/BPD/32323/2006 to C.S.]; a PhD fellowship from FCT [grant number SFRH/BD/68502/2010 to A.P.]; and a FCT grant [grant number PTDC/SAU-OBD/103981/2008 to S.L.].

PY - 2014/6/15

Y1 - 2014/6/15

N2 - The Arf-like protein Arl13b has been implicated in ciliogenesis and Sonic hedgehog signaling. Furthermore, we have previously shown that it regulates endocytic recycling traffic and interacts with actin. Herein, we report that the non-muscle myosin heavy chain IIA, also known as Myh9, is an Arl13b effector. Moreover, we found that both proteins localized to circular dorsal ruffles (CDRs) induced by platelet-derived growth factor stimulation and are required for their formation. CDRs are ring-shaped actin-dependent structures formed on the dorsal cell surface and are involved in diverse processes, such as macropinocytosis, integrin recycling, internalization of receptor tyrosine kinases and cell migration. We found that Arl13b or Myh9 silencing impaired cell migration, suggesting that Arl13b is required for this function through the interaction with Myh9. Moreover, Arl13b silencing impaired neural crest cell migration in zebrafish embryos. Furthermore, we showed that Arl13b is required for the formation of CDRs in migrating cells. Thus, our results indicate a new role for Arl13b in actin cytoskeleton remodeling through the interaction with Myh9, by driving the formation of CDRs necessary for cell migration.

AB - The Arf-like protein Arl13b has been implicated in ciliogenesis and Sonic hedgehog signaling. Furthermore, we have previously shown that it regulates endocytic recycling traffic and interacts with actin. Herein, we report that the non-muscle myosin heavy chain IIA, also known as Myh9, is an Arl13b effector. Moreover, we found that both proteins localized to circular dorsal ruffles (CDRs) induced by platelet-derived growth factor stimulation and are required for their formation. CDRs are ring-shaped actin-dependent structures formed on the dorsal cell surface and are involved in diverse processes, such as macropinocytosis, integrin recycling, internalization of receptor tyrosine kinases and cell migration. We found that Arl13b or Myh9 silencing impaired cell migration, suggesting that Arl13b is required for this function through the interaction with Myh9. Moreover, Arl13b silencing impaired neural crest cell migration in zebrafish embryos. Furthermore, we showed that Arl13b is required for the formation of CDRs in migrating cells. Thus, our results indicate a new role for Arl13b in actin cytoskeleton remodeling through the interaction with Myh9, by driving the formation of CDRs necessary for cell migration.

KW - Actin cytoskeleton

KW - LOCALIZATION

KW - Arl13b

KW - PROTEINS

KW - Wound healing

KW - MACROPINOCYTOSIS

KW - Circular dorsal ruffle

KW - Small G protein

KW - ZEBRAFISH

KW - Platelet-derived growth factor signaling

KW - PRIMARY CILIA

KW - Myosin heavy chain IIA

KW - ACTIN

KW - Myh9

KW - LEAD

KW - JOUBERT-SYNDROME

KW - RECEPTOR

KW - ROLES

KW - Arl13b

KW - Myosin heavy chain IIA

KW - Myh9

KW - Circular dorsal ruffle

KW - Small G protein

KW - Actin cytoskeleton

KW - Platelet-derived growth factor signaling

KW - Wound healing

U2 - 10.1242/jcs.143446

DO - 10.1242/jcs.143446

M3 - Article

VL - 127

SP - 2709

EP - 2722

JO - Journal Of Cell Science

JF - Journal Of Cell Science

SN - 0021-9533

IS - 12

ER -