Application of kDNA as a molecular marker to analyse Leishmania infantum diversity in Portugal

Sofia Cortes; Isabel Mauricio; Ana Almeida; José Manuel Cristovão; Francine Pratlong; Jean-Pierre Dedet; Lenea Campino

doi:10.1016/j.parint.2006.07.003

Application of kDNA as a molecular marker to analyse Leishmania infantum diversity in Portugal

Sofia Cortes, Isabel Mauricio, Ana Almeida, José Manuel Cristovão, Francine Pratlong, Jean-Pierre Dedet, Lenea Campino

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Around the Mediterranean basin Leishmania infantum is an important parasite causing canine leishmaniasis and visceral and cutaneous clinical forms in both immunocompetent and immunocompromised humans. Efficient monitoring and evaluation of epidemiology with discriminatory molecular markers are required. We investigated the genetic diversity of L. infantum in Portugal by polymerase chain amplification and restriction fragment length polymorphism analysis of kinetoplastid DNA, as molecular marker. We analysed 120 Portuguese isolates of L. infantum plus 16 other non-Portuguese isolates (as a reference group) from humans, dogs and sand flies. The Portuguese population showed a high degree of polymorphism with a total of 13 profiles identified. The predominant profile was A, which was only detected in the Portuguese samples. The kinetoplastid DNA PCR-RFLP assay described here was suitable for use directly with biological samples and the profiles obtained were stable during long-term growth in vitro and in laboratory animals.

Original language	English
Pages (from-to)	277-283
Number of pages	7
Journal	Parasitology International
Volume	55
Issue number	4
DOIs	https://doi.org/10.1016/j.parint.2006.07.003
Publication status	Published - Dec 2006

Keywords

Animals
DNA, Kinetoplast
Dog Diseases
Dogs
Genetic Markers
Genetic Variation
Genotype
Geography
Humans
Insect Vectors
Leishmania infantum
Leishmaniasis, Visceral
Phlebotomus
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Portugal
Restriction Mapping
Journal Article
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.parint.2006.07.003

Cite this

@article{10a7296430b0490283c981dbc56a2b85,

title = "Application of kDNA as a molecular marker to analyse Leishmania infantum diversity in Portugal",

abstract = "Around the Mediterranean basin Leishmania infantum is an important parasite causing canine leishmaniasis and visceral and cutaneous clinical forms in both immunocompetent and immunocompromised humans. Efficient monitoring and evaluation of epidemiology with discriminatory molecular markers are required. We investigated the genetic diversity of L. infantum in Portugal by polymerase chain amplification and restriction fragment length polymorphism analysis of kinetoplastid DNA, as molecular marker. We analysed 120 Portuguese isolates of L. infantum plus 16 other non-Portuguese isolates (as a reference group) from humans, dogs and sand flies. The Portuguese population showed a high degree of polymorphism with a total of 13 profiles identified. The predominant profile was A, which was only detected in the Portuguese samples. The kinetoplastid DNA PCR-RFLP assay described here was suitable for use directly with biological samples and the profiles obtained were stable during long-term growth in vitro and in laboratory animals.",

keywords = "Animals, DNA, Kinetoplast, Dog Diseases, Dogs, Genetic Markers, Genetic Variation, Genotype, Geography, Humans, Insect Vectors, Leishmania infantum, Leishmaniasis, Visceral, Phlebotomus, Phylogeny, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Portugal, Restriction Mapping, Journal Article, Research Support, Non-U.S. Gov't",

author = "Sofia Cortes and Isabel Mauricio and Ana Almeida and Cristov{\~a}o, {Jos{\'e} Manuel} and Francine Pratlong and Jean-Pierre Dedet and Lenea Campino",

year = "2006",

month = dec,

doi = "10.1016/j.parint.2006.07.003",

language = "English",

volume = "55",

pages = "277--283",

journal = "Parasitology International",

issn = "1383-5769",

publisher = "Elsevier Science B.V., Amsterdam.",

number = "4",

}

TY - JOUR

T1 - Application of kDNA as a molecular marker to analyse Leishmania infantum diversity in Portugal

AU - Cortes, Sofia

AU - Mauricio, Isabel

AU - Almeida, Ana

AU - Cristovão, José Manuel

AU - Pratlong, Francine

AU - Dedet, Jean-Pierre

AU - Campino, Lenea

PY - 2006/12

Y1 - 2006/12

N2 - Around the Mediterranean basin Leishmania infantum is an important parasite causing canine leishmaniasis and visceral and cutaneous clinical forms in both immunocompetent and immunocompromised humans. Efficient monitoring and evaluation of epidemiology with discriminatory molecular markers are required. We investigated the genetic diversity of L. infantum in Portugal by polymerase chain amplification and restriction fragment length polymorphism analysis of kinetoplastid DNA, as molecular marker. We analysed 120 Portuguese isolates of L. infantum plus 16 other non-Portuguese isolates (as a reference group) from humans, dogs and sand flies. The Portuguese population showed a high degree of polymorphism with a total of 13 profiles identified. The predominant profile was A, which was only detected in the Portuguese samples. The kinetoplastid DNA PCR-RFLP assay described here was suitable for use directly with biological samples and the profiles obtained were stable during long-term growth in vitro and in laboratory animals.

AB - Around the Mediterranean basin Leishmania infantum is an important parasite causing canine leishmaniasis and visceral and cutaneous clinical forms in both immunocompetent and immunocompromised humans. Efficient monitoring and evaluation of epidemiology with discriminatory molecular markers are required. We investigated the genetic diversity of L. infantum in Portugal by polymerase chain amplification and restriction fragment length polymorphism analysis of kinetoplastid DNA, as molecular marker. We analysed 120 Portuguese isolates of L. infantum plus 16 other non-Portuguese isolates (as a reference group) from humans, dogs and sand flies. The Portuguese population showed a high degree of polymorphism with a total of 13 profiles identified. The predominant profile was A, which was only detected in the Portuguese samples. The kinetoplastid DNA PCR-RFLP assay described here was suitable for use directly with biological samples and the profiles obtained were stable during long-term growth in vitro and in laboratory animals.

KW - Animals

KW - DNA, Kinetoplast

KW - Dog Diseases

KW - Dogs

KW - Genetic Markers

KW - Genetic Variation

KW - Genotype

KW - Geography

KW - Humans

KW - Insect Vectors

KW - Leishmania infantum

KW - Leishmaniasis, Visceral

KW - Phlebotomus

KW - Phylogeny

KW - Polymerase Chain Reaction

KW - Polymorphism, Restriction Fragment Length

KW - Portugal

KW - Restriction Mapping

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-33750347655&origin=inward&txGid=6d3b3af0051afcd67fe967834f678bcc

UR - https://www.sciencedirect.com/science/article/abs/pii/S1383576906001000?via%3Dihub

U2 - 10.1016/j.parint.2006.07.003

DO - 10.1016/j.parint.2006.07.003

M3 - Article

C2 - 16959531

SN - 1383-5769

VL - 55

SP - 277

EP - 283

JO - Parasitology International

JF - Parasitology International

IS - 4

ER -

Application of kDNA as a molecular marker to analyse Leishmania infantum diversity in Portugal

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this