Application of hyperthermia for cancer treatment: Synthesis and characterization of magnetic nanoparticles and their internalization on tumor cell lines

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Truncated sialylated O-glycans, such as cell-surface carbohydrate antigen sialyl-Tn (STn) are overexpressed by several cancer types, but not by the respective normal tissues. STn expression is associated with oncogenesis and metastatic ability of cancer cells, with reduced overall survival and lack of response to chemotherapy. Advances in nanomedicine have resulted in rapid development of biocompatible superparamagnetic iron oxide nanoparticles (SPIONs) with considerable potential in cancer treatment. Therefore, in this study SPIONs coated with oleic acid (OA) or dimercaptosuccinic acid (DMSA) were developed and characterized for internalization in two breast cancer cell lines: cell line expressing the STn antigen and the corresponding control. SPIONs with an average diameter of 8 nm showed superparamagnetic behavior and high potential to be used as magnetic hyperthermia agents. OA and DMSA coating provided high stability of SPIONs in physiological conditions while not changing their main properties. NPs internalization studies showed a higher accumulation of DMSA coated NPs in the breast cancer MDA-MB-231 WT cell line. In MDA-MB-231 cell line expressing STn both coated NPs showed a similar accumulation. Therefore, STn antigen can act as a receptor capable of detecting and covalently bind to the molecules present on NPs surface and induce their cellular uptake by endocytosis.

Original languageEnglish
Title of host publication6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538685068
DOIs
Publication statusPublished - 15 Apr 2019
Event6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Lisbon, Portugal
Duration: 22 Feb 201923 Feb 2019

Conference

Conference6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019
CountryPortugal
CityLisbon
Period22/02/1923/02/19

Fingerprint

Oncology
Tumors
Succimer
Iron oxides
Cells
Nanoparticles
Antigens
Oleic acid
Oleic Acid
Acids
Medical nanotechnology
Chemotherapy
Carbohydrates
Polysaccharides
Tissue
Coatings
Molecules
ferric oxide
sialosyl-Tn antigen

Cite this

@inproceedings{827763d6ae4b465daaa9e47232f2e170,
title = "Application of hyperthermia for cancer treatment: Synthesis and characterization of magnetic nanoparticles and their internalization on tumor cell lines",
abstract = "Truncated sialylated O-glycans, such as cell-surface carbohydrate antigen sialyl-Tn (STn) are overexpressed by several cancer types, but not by the respective normal tissues. STn expression is associated with oncogenesis and metastatic ability of cancer cells, with reduced overall survival and lack of response to chemotherapy. Advances in nanomedicine have resulted in rapid development of biocompatible superparamagnetic iron oxide nanoparticles (SPIONs) with considerable potential in cancer treatment. Therefore, in this study SPIONs coated with oleic acid (OA) or dimercaptosuccinic acid (DMSA) were developed and characterized for internalization in two breast cancer cell lines: cell line expressing the STn antigen and the corresponding control. SPIONs with an average diameter of 8 nm showed superparamagnetic behavior and high potential to be used as magnetic hyperthermia agents. OA and DMSA coating provided high stability of SPIONs in physiological conditions while not changing their main properties. NPs internalization studies showed a higher accumulation of DMSA coated NPs in the breast cancer MDA-MB-231 WT cell line. In MDA-MB-231 cell line expressing STn both coated NPs showed a similar accumulation. Therefore, STn antigen can act as a receptor capable of detecting and covalently bind to the molecules present on NPs surface and induce their cellular uptake by endocytosis.",
author = "Chaparro, {Catarina I. P.} and Loureiro, {Liliana R.} and Valente, {Manuel Almeida} and Videira, {Paula A.} and Borges, {Jo{\~a}o Paulo} and Soares, {Paula I. P.}",
note = "Research supported by National Funds through FCT - Portuguese Foundation for Science and Technology, Reference UID/CTM/50025/2013, FEDER funds through the COMPETE 2020 Program under the project number POCI-01-0145-FEDER-007688. This work was also funded by the Scientific merit prize Santander-Totta - Lisbon New University - {"}Antibody engineering for breast cancer therapy{"} 2013. Catarina I. P. Chaparro also acknowledges the financial support from Liga Portuguesa Contra o Cancro (LPCC)/Pfizer 2017.",
year = "2019",
month = "4",
day = "15",
doi = "10.1109/ENBENG.2019.8692485",
language = "English",
booktitle = "6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
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}

Chaparro, CIP, Loureiro, LR, Valente, MA, Videira, PA, Borges, JP & Soares, PIP 2019, Application of hyperthermia for cancer treatment: Synthesis and characterization of magnetic nanoparticles and their internalization on tumor cell lines. in 6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings., 8692485, Institute of Electrical and Electronics Engineers Inc., 6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019, Lisbon, Portugal, 22/02/19. https://doi.org/10.1109/ENBENG.2019.8692485

Application of hyperthermia for cancer treatment: Synthesis and characterization of magnetic nanoparticles and their internalization on tumor cell lines. / Chaparro, Catarina I. P.; Loureiro, Liliana R.; Valente, Manuel Almeida; Videira, Paula A.; Borges, João Paulo; Soares, Paula I. P.

6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2019. 8692485.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Application of hyperthermia for cancer treatment: Synthesis and characterization of magnetic nanoparticles and their internalization on tumor cell lines

AU - Chaparro, Catarina I. P.

AU - Loureiro, Liliana R.

AU - Valente, Manuel Almeida

AU - Videira, Paula A.

AU - Borges, João Paulo

AU - Soares, Paula I. P.

N1 - Research supported by National Funds through FCT - Portuguese Foundation for Science and Technology, Reference UID/CTM/50025/2013, FEDER funds through the COMPETE 2020 Program under the project number POCI-01-0145-FEDER-007688. This work was also funded by the Scientific merit prize Santander-Totta - Lisbon New University - "Antibody engineering for breast cancer therapy" 2013. Catarina I. P. Chaparro also acknowledges the financial support from Liga Portuguesa Contra o Cancro (LPCC)/Pfizer 2017.

PY - 2019/4/15

Y1 - 2019/4/15

N2 - Truncated sialylated O-glycans, such as cell-surface carbohydrate antigen sialyl-Tn (STn) are overexpressed by several cancer types, but not by the respective normal tissues. STn expression is associated with oncogenesis and metastatic ability of cancer cells, with reduced overall survival and lack of response to chemotherapy. Advances in nanomedicine have resulted in rapid development of biocompatible superparamagnetic iron oxide nanoparticles (SPIONs) with considerable potential in cancer treatment. Therefore, in this study SPIONs coated with oleic acid (OA) or dimercaptosuccinic acid (DMSA) were developed and characterized for internalization in two breast cancer cell lines: cell line expressing the STn antigen and the corresponding control. SPIONs with an average diameter of 8 nm showed superparamagnetic behavior and high potential to be used as magnetic hyperthermia agents. OA and DMSA coating provided high stability of SPIONs in physiological conditions while not changing their main properties. NPs internalization studies showed a higher accumulation of DMSA coated NPs in the breast cancer MDA-MB-231 WT cell line. In MDA-MB-231 cell line expressing STn both coated NPs showed a similar accumulation. Therefore, STn antigen can act as a receptor capable of detecting and covalently bind to the molecules present on NPs surface and induce their cellular uptake by endocytosis.

AB - Truncated sialylated O-glycans, such as cell-surface carbohydrate antigen sialyl-Tn (STn) are overexpressed by several cancer types, but not by the respective normal tissues. STn expression is associated with oncogenesis and metastatic ability of cancer cells, with reduced overall survival and lack of response to chemotherapy. Advances in nanomedicine have resulted in rapid development of biocompatible superparamagnetic iron oxide nanoparticles (SPIONs) with considerable potential in cancer treatment. Therefore, in this study SPIONs coated with oleic acid (OA) or dimercaptosuccinic acid (DMSA) were developed and characterized for internalization in two breast cancer cell lines: cell line expressing the STn antigen and the corresponding control. SPIONs with an average diameter of 8 nm showed superparamagnetic behavior and high potential to be used as magnetic hyperthermia agents. OA and DMSA coating provided high stability of SPIONs in physiological conditions while not changing their main properties. NPs internalization studies showed a higher accumulation of DMSA coated NPs in the breast cancer MDA-MB-231 WT cell line. In MDA-MB-231 cell line expressing STn both coated NPs showed a similar accumulation. Therefore, STn antigen can act as a receptor capable of detecting and covalently bind to the molecules present on NPs surface and induce their cellular uptake by endocytosis.

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U2 - 10.1109/ENBENG.2019.8692485

DO - 10.1109/ENBENG.2019.8692485

M3 - Conference contribution

BT - 6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings

PB - Institute of Electrical and Electronics Engineers Inc.

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