Abstract
This study examined the applicability of rep-PCR genomic fingerprinting to molecular typing of Phaeoacremonium and Phaeomoniella species found on grapevines and associated with the esca syndrome. Fingerprints obtained by rep-PCR were compared with those obtained by microsatellite-primed PCR. The rep-PCR patterns clearly separated the species, and in some cases revealed intraspecific variability. Dendrograms revealed a clear genetic distance between Phaeomoniella chlamydospora and the three Phaeoacremonium species analysed. The results demonstrate that rep-PCR can be a complementary or alternative strategy to other molecular methods as a tool for identification of species and strains of these two genera.
Original language | English |
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Pages (from-to) | 629-634 |
Number of pages | 6 |
Journal | Plant Pathology |
Volume | 53 |
Issue number | 5 |
DOIs | |
Publication status | Published - Oct 2004 |
Keywords
- Esca disease
- Phaeoacremonium
- Phaeomoniella
- rep-PCR
- Vitis vinifera