Applicability of rep-PCR genomic fingerprinting to molecular discrimination of members of the genera Phaeoacremonium and Phaeomoniella

Artur Alves, Isabel S. Henriques, Sílvia Fragoeiro, Conceição Vieira Santos, Alan John Lander Phillips, António C. M. Correia

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

This study examined the applicability of rep-PCR genomic fingerprinting to molecular typing of Phaeoacremonium and Phaeomoniella species found on grapevines and associated with the esca syndrome. Fingerprints obtained by rep-PCR were compared with those obtained by microsatellite-primed PCR. The rep-PCR patterns clearly separated the species, and in some cases revealed intraspecific variability. Dendrograms revealed a clear genetic distance between Phaeomoniella chlamydospora and the three Phaeoacremonium species analysed. The results demonstrate that rep-PCR can be a complementary or alternative strategy to other molecular methods as a tool for identification of species and strains of these two genera.

Original languageEnglish
Pages (from-to)629-634
Number of pages6
JournalPlant Pathology
Volume53
Issue number5
DOIs
Publication statusPublished - Oct 2004

Keywords

  • Esca disease
  • Phaeoacremonium
  • Phaeomoniella
  • rep-PCR
  • Vitis vinifera

Fingerprint

Dive into the research topics of 'Applicability of rep-PCR genomic fingerprinting to molecular discrimination of members of the genera Phaeoacremonium and Phaeomoniella'. Together they form a unique fingerprint.

Cite this