Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems

Research output: Contribution to journalArticlepeer-review

Abstract

Reactive oxygen species (ROS) are dangerous sources of macromolecular damage. While most derive from mitochondrial oxidative phosphorylation, their production can be triggered by ex-ogenous stresses, surpassing the extinction capacity of intrinsic antioxidant defense systems of cells. Here, we report the antioxidant activity of FucoPol, a fucose-rich polyanionic polysaccharide produced by Enterobacter A47, containing ca. 17 wt% of negatively charged residues in its struc-ture. Ferric reducing antioxidant power (FRAP) assays coupled to Hill binding kinetics fitting have shown FucoPol can neutralize ferricyanide and Fe3+-TPTZ species at an EC50 of 896 and 602 µg/mL, respectively, with positive binding cooperativity (2.52 ≤ H ≤ 4.85). This reducing power is greater than most polysaccharides reported. Moreover, an optimal 0.25% w/v FucoPol concentration shown previously to be cryo-and photoprotective was also demonstrated to protect Vero cells against H2O2-induced acute exposure not only by attenuating metabolic viability decay, but also by accentuating post-stress proliferation capacity, whilst preserving cell morphology. These results on antioxidant activity provide evidence for the biopolymer’s ability to prevent positive feedback cascades of the radical-producing Fenton reaction. Ultimately, FucoPol provides a biotechnological alternative for implementation in cryopreservation, food supplementation, and photoprotective sunscreen formula design, as all fields benefit from an antioxidant functionality.
Original languageEnglish
Article number3020
JournalPolymers
Volume13
Issue number18
DOIs
Publication statusPublished - 1 Sep 2021

Keywords

  • Antioxidant
  • Cryopreservation
  • Food
  • FRAP
  • FucoPol
  • Hill
  • Hydrogen peroxide
  • Polysaccharide
  • Radiation
  • Stress

Fingerprint

Dive into the research topics of 'Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems'. Together they form a unique fingerprint.

Cite this