Antimicrobial resistance and molecular characteristics of Neisseria gonorrhoeae isolates from men who have sex with men

Calado, Joana, Rita Maria Rodrigues Teixeira Castro, Lopes, Ângela O., Campos, Maria José, Rocha, Miguel, F Pereira

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVES:
To analyze the susceptibility of Neisseria gonorrhoeae isolates to penicillin (Pen), cefixime (Cfm), ceftriaxone (Cro), tetracycline (Tet), ciprofloxacin (Cip), azithromycin (Azm), and spectinomycin (Spt), and to verify the presence of mutations in resistance genes.

METHODS:
Antibiotic susceptibility testing was performed by Etest method on 30 N. gonorrhoeae isolates collected from the MSM (men who have sex with men) population. PCR and DNA sequencing were performed to identify mutations within the penA, mtrR, gyrA, and parC genes in intermediately resistant and fully resistant isolates.

RESULTS:
N. gonorrhoeae isolates showed intermediate or full resistance to Pen (73%), Cfm (3%), Tet (60%), Cip (37%), and Azm (13%). One isolate with resistance to Cfm presented a penicillin-binding protein 2 (PBP2) mosaic XXXIV. All isolates with intermediate or full resistance to Pen (except at PBP2 mosaic) presented a D345a in PBP2. All Cip-resistant isolates had an S91F in the gyrA gene together with mutations in the parC gene. All intermediate or fully resistant isolates to substrates of the MtrCDE efflux pump had an A39T or G45D mutation in the mtrR gene or an adenine deletion within the mtrR promoter. One isolate presented a Neisseria meningitidis-like mtrR promoter sequence.

CONCLUSIONS:
The results of this study are consistent with the findings of other studies and reinforce the importance of the expedient development of new therapeutic options.
Original languageEnglish
Pages (from-to)116-122
Number of pages7
JournalInternational Journal of Infectious Diseases
VolumeVol. 79
DOIs
Publication statusPublished - Feb 2019

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Neisseria gonorrhoeae
Cefixime
Penicillin-Binding Proteins
Ciprofloxacin
Penicillin Resistance
Mutation
Azithromycin
Genes
Tetracycline
Disk Diffusion Antimicrobial Tests
Spectinomycin
Neisseria meningitidis
Ceftriaxone
Adenine
DNA Sequence Analysis
Penicillins
Anti-Bacterial Agents
Polymerase Chain Reaction
Population

Keywords

  • Antimicrobial resistance
  • Neisseria gonorrhoeae
  • gyrA
  • mtrR
  • parC
  • penA

Cite this

@article{eee2f3a1926f4106af470c3cc220195b,
title = "Antimicrobial resistance and molecular characteristics of Neisseria gonorrhoeae isolates from men who have sex with men",
abstract = "OBJECTIVES:To analyze the susceptibility of Neisseria gonorrhoeae isolates to penicillin (Pen), cefixime (Cfm), ceftriaxone (Cro), tetracycline (Tet), ciprofloxacin (Cip), azithromycin (Azm), and spectinomycin (Spt), and to verify the presence of mutations in resistance genes.METHODS:Antibiotic susceptibility testing was performed by Etest method on 30 N. gonorrhoeae isolates collected from the MSM (men who have sex with men) population. PCR and DNA sequencing were performed to identify mutations within the penA, mtrR, gyrA, and parC genes in intermediately resistant and fully resistant isolates.RESULTS:N. gonorrhoeae isolates showed intermediate or full resistance to Pen (73{\%}), Cfm (3{\%}), Tet (60{\%}), Cip (37{\%}), and Azm (13{\%}). One isolate with resistance to Cfm presented a penicillin-binding protein 2 (PBP2) mosaic XXXIV. All isolates with intermediate or full resistance to Pen (except at PBP2 mosaic) presented a D345a in PBP2. All Cip-resistant isolates had an S91F in the gyrA gene together with mutations in the parC gene. All intermediate or fully resistant isolates to substrates of the MtrCDE efflux pump had an A39T or G45D mutation in the mtrR gene or an adenine deletion within the mtrR promoter. One isolate presented a Neisseria meningitidis-like mtrR promoter sequence.CONCLUSIONS:The results of this study are consistent with the findings of other studies and reinforce the importance of the expedient development of new therapeutic options.",
keywords = "Antimicrobial resistance, Neisseria gonorrhoeae, gyrA, mtrR, parC, penA",
author = "Calado, Joana and Castro, {Rita Maria Rodrigues Teixeira} and {{\^A}ngela O.}, Lopes, and {Maria Jos{\'e}}, Campos, and Rocha, Miguel and F Pereira",
year = "2019",
month = "2",
doi = "10.1016/j.ijid.2018.10.030",
language = "English",
volume = "Vol. 79",
pages = "116--122",
journal = "International Journal of Infectious Diseases",
issn = "1201-9712",
publisher = "Elsevier Science B.V., Amsterdam.",

}

Antimicrobial resistance and molecular characteristics of Neisseria gonorrhoeae isolates from men who have sex with men. / Joana, Calado, ; Castro, Rita Maria Rodrigues Teixeira; Ângela O., Lopes, ; Maria José, Campos, ; Miguel, Rocha, ; Pereira, F.

In: International Journal of Infectious Diseases, Vol. Vol. 79, 02.2019, p. 116-122.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antimicrobial resistance and molecular characteristics of Neisseria gonorrhoeae isolates from men who have sex with men

AU - Joana, Calado,

AU - Castro, Rita Maria Rodrigues Teixeira

AU - Ângela O., Lopes,

AU - Maria José, Campos,

AU - Miguel, Rocha,

AU - Pereira, F

PY - 2019/2

Y1 - 2019/2

N2 - OBJECTIVES:To analyze the susceptibility of Neisseria gonorrhoeae isolates to penicillin (Pen), cefixime (Cfm), ceftriaxone (Cro), tetracycline (Tet), ciprofloxacin (Cip), azithromycin (Azm), and spectinomycin (Spt), and to verify the presence of mutations in resistance genes.METHODS:Antibiotic susceptibility testing was performed by Etest method on 30 N. gonorrhoeae isolates collected from the MSM (men who have sex with men) population. PCR and DNA sequencing were performed to identify mutations within the penA, mtrR, gyrA, and parC genes in intermediately resistant and fully resistant isolates.RESULTS:N. gonorrhoeae isolates showed intermediate or full resistance to Pen (73%), Cfm (3%), Tet (60%), Cip (37%), and Azm (13%). One isolate with resistance to Cfm presented a penicillin-binding protein 2 (PBP2) mosaic XXXIV. All isolates with intermediate or full resistance to Pen (except at PBP2 mosaic) presented a D345a in PBP2. All Cip-resistant isolates had an S91F in the gyrA gene together with mutations in the parC gene. All intermediate or fully resistant isolates to substrates of the MtrCDE efflux pump had an A39T or G45D mutation in the mtrR gene or an adenine deletion within the mtrR promoter. One isolate presented a Neisseria meningitidis-like mtrR promoter sequence.CONCLUSIONS:The results of this study are consistent with the findings of other studies and reinforce the importance of the expedient development of new therapeutic options.

AB - OBJECTIVES:To analyze the susceptibility of Neisseria gonorrhoeae isolates to penicillin (Pen), cefixime (Cfm), ceftriaxone (Cro), tetracycline (Tet), ciprofloxacin (Cip), azithromycin (Azm), and spectinomycin (Spt), and to verify the presence of mutations in resistance genes.METHODS:Antibiotic susceptibility testing was performed by Etest method on 30 N. gonorrhoeae isolates collected from the MSM (men who have sex with men) population. PCR and DNA sequencing were performed to identify mutations within the penA, mtrR, gyrA, and parC genes in intermediately resistant and fully resistant isolates.RESULTS:N. gonorrhoeae isolates showed intermediate or full resistance to Pen (73%), Cfm (3%), Tet (60%), Cip (37%), and Azm (13%). One isolate with resistance to Cfm presented a penicillin-binding protein 2 (PBP2) mosaic XXXIV. All isolates with intermediate or full resistance to Pen (except at PBP2 mosaic) presented a D345a in PBP2. All Cip-resistant isolates had an S91F in the gyrA gene together with mutations in the parC gene. All intermediate or fully resistant isolates to substrates of the MtrCDE efflux pump had an A39T or G45D mutation in the mtrR gene or an adenine deletion within the mtrR promoter. One isolate presented a Neisseria meningitidis-like mtrR promoter sequence.CONCLUSIONS:The results of this study are consistent with the findings of other studies and reinforce the importance of the expedient development of new therapeutic options.

KW - Antimicrobial resistance

KW - Neisseria gonorrhoeae

KW - gyrA

KW - mtrR

KW - parC

KW - penA

U2 - 10.1016/j.ijid.2018.10.030

DO - 10.1016/j.ijid.2018.10.030

M3 - Article

VL - Vol. 79

SP - 116

EP - 122

JO - International Journal of Infectious Diseases

JF - International Journal of Infectious Diseases

SN - 1201-9712

ER -