Lavandin (Lavandula hybrida) essential oil contains components with biocide and antiviral properties that can be used as substitutes of antibiotics. This application requires an appropriate formulation of the essential oil. In the present work, the antimicrobial activity of free and encapsulated lavandin essential oil against three pathogenic bacteria (Gram-negative: Escherichia con; Gram-positive: Staphylococcus aureus and Bacillus cereus) was determined. The formulations were prepared using innovative high-pressure techniques (PGSS and PGSS-drying) as well as spray-drying. Carrier materials used for the encapsulation were soybean lecithin, n-octenyl succinic anhydride (OSA) modified starch and poly-caprolactone. Results demonstrated that lavandin oil antibacterial activity could be enhanced by encapsulation, due to the protection and control release of the oil. As well, encapsulation might present an interesting opportunity to facilitate the action of antimicrobials, improving essential oil penetration inside of the outer membrane. (C) 2012 Elsevier B.V. All rights reserved.