Abstract

Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic joint inflammation and one of the main causes of chronic disability worldwide with high prevalence in the ageing population. RA is characterized by autoantibody production, synovial inflammation and bone destruction, and the most accepted biomarker is rheumatoid factor (RF) autoantibodies. In this work, we developed a low-cost approach for the detection and quantification of the RF marker. This colorimetric immunosensor is based on gold nanoprobe crosslinking that results in extensive aggregation in the presence of the pentameric IgM RF. Aggregation of the nanoconjugates yields a color change from red to purple that can be easily observed by the naked eye. The interaction between nanoconjugates and the specific target was confirmed via dynamic light scattering (DLS), Raman spectroscopy and atomic force microscopy (AFM) imaging. This conceptual system shows a LOD of 4.15 UA mL-1 IgM RF (clinical threshold is set for 20 IU mL-1). The one-step biosensor strategy herein proposed is much faster than conventional detection techniques, without the need for secondary antibodies, additional complex washing or signal amplification protocols. To the best of our knowledge this is the first report on target induced aggregation of gold nanoprobes for quantitative colorimetric autoantibody detection.

Original languageEnglish
Pages (from-to)3613-3619
Number of pages7
JournalAnalyst
Volume144
Issue number11
Early online date9 May 2019
DOIs
Publication statusPublished - 7 Jun 2019

Fingerprint

Rheumatoid Factor
Nanoconjugates
Antibodies
Gold
Nanoparticles
Nanoprobes
antibody
Rheumatoid Arthritis
Screening
Agglomeration
gold
Autoantibodies
Immunoglobulin M
Immunosensors
aging population
atomic force microscopy
disability
Raman spectroscopy
Biomarkers
light scattering

Cite this

@article{0c876e0651ce451097e65dc8c6ca390b,
title = "Antibody modified gold nanoparticles for fast colorimetric screening of rheumatoid arthritis",
abstract = "Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic joint inflammation and one of the main causes of chronic disability worldwide with high prevalence in the ageing population. RA is characterized by autoantibody production, synovial inflammation and bone destruction, and the most accepted biomarker is rheumatoid factor (RF) autoantibodies. In this work, we developed a low-cost approach for the detection and quantification of the RF marker. This colorimetric immunosensor is based on gold nanoprobe crosslinking that results in extensive aggregation in the presence of the pentameric IgM RF. Aggregation of the nanoconjugates yields a color change from red to purple that can be easily observed by the naked eye. The interaction between nanoconjugates and the specific target was confirmed via dynamic light scattering (DLS), Raman spectroscopy and atomic force microscopy (AFM) imaging. This conceptual system shows a LOD of 4.15 UA mL-1 IgM RF (clinical threshold is set for 20 IU mL-1). The one-step biosensor strategy herein proposed is much faster than conventional detection techniques, without the need for secondary antibodies, additional complex washing or signal amplification protocols. To the best of our knowledge this is the first report on target induced aggregation of gold nanoprobes for quantitative colorimetric autoantibody detection.",
author = "Bruno Veigas and Ana Matias and Tom{\'a}s Calmeiro and Elvira Fortunato and Fernandes, {Alexandra R.} and Baptista, {Pedro Viana}",
note = "info:eu-repo/grantAgreement/FCT/5876/147258/PT# POCI-01-0145-FEDER-007728. Project INNO INDIGO 2015 - RA Detect: One Platform-Multiple biomarker detection of Rheumatoid Arthritis (https://radetect.eu/). SFRH/BPD/124311/2016. Sem PDF conforme despacho.",
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T1 - Antibody modified gold nanoparticles for fast colorimetric screening of rheumatoid arthritis

AU - Veigas, Bruno

AU - Matias, Ana

AU - Calmeiro, Tomás

AU - Fortunato, Elvira

AU - Fernandes, Alexandra R.

AU - Baptista, Pedro Viana

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PY - 2019/6/7

Y1 - 2019/6/7

N2 - Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic joint inflammation and one of the main causes of chronic disability worldwide with high prevalence in the ageing population. RA is characterized by autoantibody production, synovial inflammation and bone destruction, and the most accepted biomarker is rheumatoid factor (RF) autoantibodies. In this work, we developed a low-cost approach for the detection and quantification of the RF marker. This colorimetric immunosensor is based on gold nanoprobe crosslinking that results in extensive aggregation in the presence of the pentameric IgM RF. Aggregation of the nanoconjugates yields a color change from red to purple that can be easily observed by the naked eye. The interaction between nanoconjugates and the specific target was confirmed via dynamic light scattering (DLS), Raman spectroscopy and atomic force microscopy (AFM) imaging. This conceptual system shows a LOD of 4.15 UA mL-1 IgM RF (clinical threshold is set for 20 IU mL-1). The one-step biosensor strategy herein proposed is much faster than conventional detection techniques, without the need for secondary antibodies, additional complex washing or signal amplification protocols. To the best of our knowledge this is the first report on target induced aggregation of gold nanoprobes for quantitative colorimetric autoantibody detection.

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