TY - JOUR
T1 - Antibody-drug gold nanoantennas with Raman spectroscopic fingerprints for in vivo tumour theranostics
AU - Conde, João
AU - Bao, Chenchen
AU - Cui, Daxiang
AU - Baptista, Pedro V.
AU - Tian, Furong
N1 - SFRH/BD/62957/2009
PEst-OE/SAU/UI0009/2011
Sem PDF conforme despacho.
PY - 2014/6/10
Y1 - 2014/6/10
N2 - Inspired by the ability of SERS nanoantennas to provide an integrated platform to enhance disease targeting in vivo, we developed a highly sensitive probe for in vivo tumour recognition with the capacity to target specific cancer biomarkers such as epidermal growth factor receptors (EGFR) on human cancer cells and xenograft tumour models. Here, we used ~ 90 nm gold nanoparticles capped by a Raman reporter, encapsulated and entrapped by larger polymers and a FDA antibody-drug conjugate - Cetuximab (Erbitux®) - that specifically targets EGFR and turns off a main signalling cascade for cancer cells to proliferate and survive. These drug/SERS gold nanoantennas present a high Raman signal both in cancer cells and in mice bearing xenograft tumours. Moreover, the Raman detection signal is accomplished simultaneously by extensive tumour growth inhibition in mice, making these gold nanoantennas ideal for cancer nanotheranostics, i.e. tumour detection and tumour cell inhibition at the same time.
AB - Inspired by the ability of SERS nanoantennas to provide an integrated platform to enhance disease targeting in vivo, we developed a highly sensitive probe for in vivo tumour recognition with the capacity to target specific cancer biomarkers such as epidermal growth factor receptors (EGFR) on human cancer cells and xenograft tumour models. Here, we used ~ 90 nm gold nanoparticles capped by a Raman reporter, encapsulated and entrapped by larger polymers and a FDA antibody-drug conjugate - Cetuximab (Erbitux®) - that specifically targets EGFR and turns off a main signalling cascade for cancer cells to proliferate and survive. These drug/SERS gold nanoantennas present a high Raman signal both in cancer cells and in mice bearing xenograft tumours. Moreover, the Raman detection signal is accomplished simultaneously by extensive tumour growth inhibition in mice, making these gold nanoantennas ideal for cancer nanotheranostics, i.e. tumour detection and tumour cell inhibition at the same time.
KW - Antibody-drug conjugates
KW - In vivo tumour targeting
KW - Nanotheranostics
KW - Raman fingerprints
KW - SERS
UR - http://www.scopus.com/inward/record.url?scp=84898606435&partnerID=8YFLogxK
U2 - 10.1016/j.jconrel.2014.03.045
DO - 10.1016/j.jconrel.2014.03.045
M3 - Article
C2 - 24704711
AN - SCOPUS:84898606435
SN - 0168-3659
VL - 183
SP - 87
EP - 93
JO - Journal of Controlled Release
JF - Journal of Controlled Release
IS - 1
ER -