Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1485-1494 |
| Number of pages | 10 |
| Journal | Annals Of Microbiology |
| Volume | 62 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Dec 2012 |
Keywords
- Antibiotic resistance
- Enterococci
- Resistance genes
- Animalia
- Bos
- Enterococcus
- Enterococcus faecium
- Enterococcus hirae
- Ovis aries
- Suidae
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Antibiotic resistance and mechanisms implicated in fecal enterococci recovered from pigs, cattle and sheep in a Portuguese slaughterhouse. / Ramos, S.; Igrejas, Gilberto; Capelo, José Luis; Poeta, Patrícia Alexandra Curado Quintas Dinis.
In: Annals Of Microbiology, Vol. 62, No. 4, 12.2012, p. 1485-1494.Research output: Contribution to journal › Article
TY - JOUR
T1 - Antibiotic resistance and mechanisms implicated in fecal enterococci recovered from pigs, cattle and sheep in a Portuguese slaughterhouse
AU - Ramos, S.
AU - Igrejas, Gilberto
AU - Capelo, José Luis
AU - Poeta, Patrícia Alexandra Curado Quintas Dinis
N1 - Cited By :5 Export Date: 7 May 2017 Correspondence Address: Poeta, P.; Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal; email: ppoeta@utad.pt References: Aarestrup, F.M., Agerso, Y., Gerner-Smidt, P., Madsen, M., Jensen, L.B., Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark Diagnostic Microbiology and Infectious Disease, 37 (2), pp. 127-137. , DOI 10.1016/S0732-8893(00)00130-9, PII S0732889300001309; Aarestrup, F.M., Hasman, H., Jensen, L.B., Moreno, M., Herrero, I.A., Dominguez, L., Finn, M., Franklin, A., Antimicrobial resistance among enterococci from pigs in three European countries Applied and Environmental Microbiology, 68 (8), pp. 4127-4129. , DOI 10.1128/AEM.68.8.4127-4129.2002; Agerso, Y., Pedersen, A.G., Aarestrup, F.M., Identification of Tn5397-like and Tn916-like transposons and diversity of the tetracycline resistance gene tet(M) in enterococci from humans, pigs and poultry J Antimicrob Chemother, 57, pp. 5832-5839. , doi:10.1093/jac/dkl069; Anderson, J.F., Parrish, T.D., Akhtar, M., Zurek, L., Hirt, H., Antibiotic resistance of enterococci in American bison (Bison bison) from a nature preserve compared to that of enterococci in pastured cattle Applied and Environmental Microbiology, 74 (6), pp. 1726-1730. , DOI 10.1128/AEM.02164-07; Araujo, C., Torres, C., Silva, N., Carneiro, C., Goncalves, A., Radhouani, H., Correia, S., Igrejas, G., Vancomycin-resistant enterococci from Portuguese wastewater treatment plants J Basic Microbiol, , doi:10.1002/jobm.201000102; Arias, C.A., Robredo, B., Singh, K.V., Torres, C., Panesso, D., Murray, B.E., Rapid identification of Enterococcus hirae and Enterococ-cus durans by PCR and detection of a homologue of the E. hirae mur-2 Gene in E. durans J Clin Microbiol, 44, pp. 41567-41570. , doi:10.1128/JCM.44.4.1567-1570.2006; Butaye, P., Devriese, L.A., Haesebrouck, F., Differences in antibiotic resistance patterns of Enterococcus faecalis and Enterococcus faecium strains isolated from farm and pet animals Antimicrobial Agents and Chemotherapy, 45 (5), pp. 1374-1378. , DOI 10.1128/AAC.45.5.1374-1378.2001; Performance Standards for Antimicrobial Susceptibility Testing 17th Edn, , C.L.S.I. 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Food-borne Pathog Dis, 7 (10), pp. 1137-1146. , doi:10.1089/fpd.2010.0552; Hayes, J.R., English, L.L., Carter, P.J., Proescholdt, T., Lee, K.Y., Wagner, D.D., White, D.G., Prevalence and Antimicrobial Resistance of Enterococcus Species Isolated from Retail Meats Applied and Environmental Microbiology, 69 (12), pp. 7153-7160. , DOI 10.1128/AEM.69.12.7153-7160.2003; Hershberger, E., Oprea, S.F., Donabedian, S.M., Perri, M., Bozigar, P., Bartlett, P., Zervos, M.J., Epidemiology of antimicrobial resistance in enterococci of animal origin Journal of Antimicrobial Chemotherapy, 55 (1), pp. 127-130. , DOI 10.1093/jac/dkh508; Jackson, C.R., Fedorka-Cray, P.J., Barrett, J.B., Hiott, L.M., Woodley, T.A., Prevalence of streptogramin resistance in enterococci from animals: identification of vatD from animal sources in the USA International Journal of Antimicrobial Agents, 30 (1), pp. 60-66. , DOI 10.1016/j.ijantimicag.2007.03.010, PII S0924857907001823; Jackson, C.R., Fedorka-Cray, P.J., Davis, J.A., Barrett, J.B., Brousse, J.H., Gustafson, J., Kucher, M., Mechanisms of antimicrobial resistance and genetic relatedness among enterococci isolated from dogs and cats in the United States J Appl Microbiol, 108, pp. 62171-62179. , doi:10.1111/j.1365-2672.2009.04619.x; Jackson, C.R., Lombard, J.E., Dargatz, D.A., Fedorka-Cray, P.J., Prevalence, species distribution and antimicrobial resistance of entero-cocci isolated from US dairy cattle Lett Appl Microbiol, 52 (1), pp. 41-48. , doi:10.1111/j.1472-765X.2010.02964.x; Kuhn, I., Iversen, A., Burman, L.G., Olsson-Liljequist, B., Franklin, A., Finn, M., Aarestrup, F., Mollby, R., Comparison of enterococcal populations in animals, humans, and the environment - A European study International Journal of Food Microbiology, 88 (2-3), pp. 133-145. , DOI 10.1016/S0168-1605(03)00176-4; Kuhn, I., Iversen, A., Finn, M., Greko, C., Burman, L.G., Blanch, A.R., Vilanova, X., Mollby, R., Occurrence and relatedness of vancomycin-resistant enterococci in animals, humans, and the environment in different European regions Applied and Environmental Microbiology, 71 (9), pp. 5383-5390. , DOI 10.1128/AEM.71.9.5383-5390.2005; MacEdo, A.S., Freitas, A.R., Abreu, C., MacHado, E., Peixe, L., Sousa, J.C., Novais, C., Characterization of antibiotic resistant enterococci isolated from untreated waters for human consumption in Portugal Int J Food Microbiol, 145, pp. 1315-1319. , doi:10.1016/j.ijfoodmicro.2010.11.024; 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PY - 2012/12
Y1 - 2012/12
N2 - A total of 194 enterococcal isolates were recovered from 198 fecal samples of pigs, cattle, and sheep obtained in a Portuguese slaughterhouse. The enterococcal species most prevalent were Enterococcus faecium and E. hirae. High percentages of resistance were detected for tetracycline in pig isolates (95.7%), sheep isolates (76.7%), and cattle isolates (49%); erythromycin resistancewas higher in pig isolates than in cattle or sheep isolates. Intermediate level of resistance was obtained to quinupristin/dalfopristin in all animal isolates (15.1-23.5%). High-level resistance to aminoglycosides was detected, HLR-S and-K was higher in pig isolates (44.3 and 32.9%, respectively) compared with cattle or sheep isolates, and modest percentages of HLR-G were obtained in pig and cattle isolates (7.1 and 3.9%, respectively). The aac(6̀)-aph (2"), aph(3̀)-IIIa, ant(6)-Ia, cat(A), erm(B), and tet(M) genes were demonstrated in most of the gentamycin-, kanamycin-, streptomycin-, chloramphenicol-, erythromycin-and tetracycline-resistant isolates, respectively. The association between the tet(M) gene and Tn916/Tn1545-like or Tn5397-like transposons was detected in 30.8 and 11.2% of the isolates, respectively. Food animals could be a reservoir for antibiotic resistance genes, and slaughterhouse cross-contamination of animals carcasses may be a food safety risk. © 2012 Springer-Verlag and the University of Milan.
AB - A total of 194 enterococcal isolates were recovered from 198 fecal samples of pigs, cattle, and sheep obtained in a Portuguese slaughterhouse. The enterococcal species most prevalent were Enterococcus faecium and E. hirae. High percentages of resistance were detected for tetracycline in pig isolates (95.7%), sheep isolates (76.7%), and cattle isolates (49%); erythromycin resistancewas higher in pig isolates than in cattle or sheep isolates. Intermediate level of resistance was obtained to quinupristin/dalfopristin in all animal isolates (15.1-23.5%). High-level resistance to aminoglycosides was detected, HLR-S and-K was higher in pig isolates (44.3 and 32.9%, respectively) compared with cattle or sheep isolates, and modest percentages of HLR-G were obtained in pig and cattle isolates (7.1 and 3.9%, respectively). The aac(6̀)-aph (2"), aph(3̀)-IIIa, ant(6)-Ia, cat(A), erm(B), and tet(M) genes were demonstrated in most of the gentamycin-, kanamycin-, streptomycin-, chloramphenicol-, erythromycin-and tetracycline-resistant isolates, respectively. The association between the tet(M) gene and Tn916/Tn1545-like or Tn5397-like transposons was detected in 30.8 and 11.2% of the isolates, respectively. Food animals could be a reservoir for antibiotic resistance genes, and slaughterhouse cross-contamination of animals carcasses may be a food safety risk. © 2012 Springer-Verlag and the University of Milan.
KW - Antibiotic resistance
KW - Enterococci
KW - Resistance genes
KW - Animalia
KW - Bos
KW - Enterococcus
KW - Enterococcus faecium
KW - Enterococcus hirae
KW - Ovis aries
KW - Suidae
U2 - 10.1007/s13213-011-0402-7
DO - 10.1007/s13213-011-0402-7
M3 - Article
VL - 62
SP - 1485
EP - 1494
JO - Annals Of Microbiology
JF - Annals Of Microbiology
SN - 1590-4261
IS - 4
ER -