Anti-inflammatory effect of rosmarinic acid and an extract of rosmarinus officinalis in rat models of local and systemic inflammation

Joao Rocha, Maria Eduardo-Figueira, Andreia Barateiro, Adelaide Fernandes, Dora Brites, Maria do Rosário Bronze, Catarina Mm Duarte, Ana Teresa Serra, Rui Pinto, Marisa Freitas, Eduarda Fernandes, Beatriz Silva-Lima, Helder Mota-Filipe, Bruno Sepodes

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Abstract

Rosmarinic acid is a polyphenolic compound and main constituent of Rosmarinus officinalis and has been shown to possess antioxidant and anti-inflammatory properties. We aimed to evaluate the anti-inflammatory properties of rosmarinic acid and of an extract of R. officinalis in local inflammation (carrageenin-induced paw oedema model in the rat), and further evaluate the protective effect of rosmarinic acid in rat models of systemic inflammation: liver ischaemia-reperfusion (I/R) and thermal injury models. In the local inflammation model, rosmarinic acid was administered at 10, 25 and 50 mg/kg (p.o.), and the extract was administered at 10 and 25 mg/kg (equivalent doses to rosmarinic acid groups) to male Wistar rats. Administration of rosmarinic acid and extract at the dose of 25 mg/kg reduced paw oedema at 6 hr by over 60%, exhibiting a dose-response effect, suggesting that rosmarinic was the main contributor to the anti-inflammatory effect. In the liver I/R model, rosmarinic acid was administered at 25 mg/kg (i.v.) 30 min. prior to the induction of ischaemia and led to the significant reduction in the serum concentration of transaminases (AST and ALT) and LDH. In the thermal injury model, rosmarinic acid was administered at 25 mg/kg (i.v.) 5 min. prior to the induction of injury and significantly reduced multi-organ dysfunction markers (liver, kidney, lung) by modulating NF-κB and metalloproteinase-9. For the first time, the anti-inflammatory potential of rosmarinic acid has been identified, as it causes a substantial reduction in inflammation, and we speculate that it might be useful in the pharmacological modulation of injuries associated to inflammation.

Original languageEnglish
Pages (from-to)398-413
Number of pages16
JournalBasic & clinical pharmacology & toxicology
Volume116
Issue number5
DOIs
Publication statusPublished - 1 May 2015

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Rosmarinus
Rats
Anti-Inflammatory Agents
Inflammation
Liver
Edema
Wounds and Injuries
Polyphenolic compounds
Ischemia
Hot Temperature
rosmarinic acid
Carrageenan
Metalloproteases
Transaminases
Reperfusion Injury
Reperfusion
Wistar Rats
Liver Diseases
Antioxidants
Modulation

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Rocha, Joao ; Eduardo-Figueira, Maria ; Barateiro, Andreia ; Fernandes, Adelaide ; Brites, Dora ; Bronze, Maria do Rosário ; Duarte, Catarina Mm ; Serra, Ana Teresa ; Pinto, Rui ; Freitas, Marisa ; Fernandes, Eduarda ; Silva-Lima, Beatriz ; Mota-Filipe, Helder ; Sepodes, Bruno. / Anti-inflammatory effect of rosmarinic acid and an extract of rosmarinus officinalis in rat models of local and systemic inflammation. In: Basic & clinical pharmacology & toxicology. 2015 ; Vol. 116, No. 5. pp. 398-413.
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abstract = "Rosmarinic acid is a polyphenolic compound and main constituent of Rosmarinus officinalis and has been shown to possess antioxidant and anti-inflammatory properties. We aimed to evaluate the anti-inflammatory properties of rosmarinic acid and of an extract of R. officinalis in local inflammation (carrageenin-induced paw oedema model in the rat), and further evaluate the protective effect of rosmarinic acid in rat models of systemic inflammation: liver ischaemia-reperfusion (I/R) and thermal injury models. In the local inflammation model, rosmarinic acid was administered at 10, 25 and 50 mg/kg (p.o.), and the extract was administered at 10 and 25 mg/kg (equivalent doses to rosmarinic acid groups) to male Wistar rats. Administration of rosmarinic acid and extract at the dose of 25 mg/kg reduced paw oedema at 6 hr by over 60{\%}, exhibiting a dose-response effect, suggesting that rosmarinic was the main contributor to the anti-inflammatory effect. In the liver I/R model, rosmarinic acid was administered at 25 mg/kg (i.v.) 30 min. prior to the induction of ischaemia and led to the significant reduction in the serum concentration of transaminases (AST and ALT) and LDH. In the thermal injury model, rosmarinic acid was administered at 25 mg/kg (i.v.) 5 min. prior to the induction of injury and significantly reduced multi-organ dysfunction markers (liver, kidney, lung) by modulating NF-κB and metalloproteinase-9. For the first time, the anti-inflammatory potential of rosmarinic acid has been identified, as it causes a substantial reduction in inflammation, and we speculate that it might be useful in the pharmacological modulation of injuries associated to inflammation.",
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Rocha, J, Eduardo-Figueira, M, Barateiro, A, Fernandes, A, Brites, D, Bronze, MDR, Duarte, CM, Serra, AT, Pinto, R, Freitas, M, Fernandes, E, Silva-Lima, B, Mota-Filipe, H & Sepodes, B 2015, 'Anti-inflammatory effect of rosmarinic acid and an extract of rosmarinus officinalis in rat models of local and systemic inflammation', Basic & clinical pharmacology & toxicology, vol. 116, no. 5, pp. 398-413. https://doi.org/10.1111/bcpt.12335

Anti-inflammatory effect of rosmarinic acid and an extract of rosmarinus officinalis in rat models of local and systemic inflammation. / Rocha, Joao; Eduardo-Figueira, Maria; Barateiro, Andreia; Fernandes, Adelaide; Brites, Dora; Bronze, Maria do Rosário; Duarte, Catarina Mm; Serra, Ana Teresa; Pinto, Rui; Freitas, Marisa; Fernandes, Eduarda; Silva-Lima, Beatriz; Mota-Filipe, Helder; Sepodes, Bruno.

In: Basic & clinical pharmacology & toxicology, Vol. 116, No. 5, 01.05.2015, p. 398-413.

Research output: Contribution to journalArticle

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AU - Eduardo-Figueira, Maria

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AU - Duarte, Catarina Mm

AU - Serra, Ana Teresa

AU - Pinto, Rui

AU - Freitas, Marisa

AU - Fernandes, Eduarda

AU - Silva-Lima, Beatriz

AU - Mota-Filipe, Helder

AU - Sepodes, Bruno

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