Anopheline antiplatelet protein from mosquito saliva regulates blood feeding behavior

Islam, Ashekul, Emran Talha Bin, Yamamoto, Daisuke S., Iyori, Mitsuhiro, Amelia, Fitri , Yusuf, Yenni, Yamaguchi, Ririka, Alam, Shah , H Silveira, Yoshida, Shigeto

Research output: Contribution to conferencePaper

Abstract

The saliva of hematophagous arthropods is enriched with a complex mixture of antihemostatic molecules, the biological functions of which are largely unknown. Anopheline antiplatelet protein (AAPP) from malaria vector mosquito exhibits strong antiplatelet activity when bound directly to host collagen by its C-terminus and through its N-terminus with Ca2+-binding activity. To investigate the biological functions of AAPP in blood feeding behavior and malaria transmission, we generated transgenic Anopheles stephensi mosquito lines expressing anti-AAPP antibody single-chain fragment (scFv) in their salivary glands. The AAPP-specific collagen-binding activity was completely abolished by AAPP-scFv complex formation in the saliva. Probing and prediuresis time, feeding success, blood meal size, and fecundity, which are all fitness characteristics, were significantly reduced in the transgenic mosquitoes. However, oocysts number in these mosquitoes were not significantly reduced following blood meal intake from Plasmodium berghei-infected mice. These results show that although AAPP plays an important role in mosquito blood feeding, its neutralizing activity did not affect sporogonic development in our laboratory model, but its high fitness cost would pose a survival risk for parasite-infected mosquitoes in nature.
Original languageEnglish
Pages3129-3140
Number of pages11
DOIs
Publication statusPublished - 28 Feb 2019

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Feeding Behavior
Culicidae
Saliva
Proteins
Malaria
Meals
Collagen
Plasmodium berghei
Single-Chain Antibodies
Anopheles
Oocysts
Arthropods
Salivary Glands
Complex Mixtures
Fertility
Blood Proteins
Parasites
Costs and Cost Analysis

Cite this

Ashekul, I., Talha Bin, E., Daisuke S., Y., Mitsuhiro, I., Fitri , A., Yenni, Y., ... Shigeto , Y. (2019). Anopheline antiplatelet protein from mosquito saliva regulates blood feeding behavior. 3129-3140. https://doi.org/10.1038/s41598-019-39960-2
Ashekul, Islam, ; Talha Bin, Emran ; Daisuke S., Yamamoto, ; Mitsuhiro, Iyori, ; Fitri , Amelia, ; Yenni, Yusuf, ; Ririka, Yamaguchi, ; Shah , Alam, ; Silveira, H ; Shigeto , Yoshida,. / Anopheline antiplatelet protein from mosquito saliva regulates blood feeding behavior. 11 p.
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Ashekul, I, Talha Bin, E, Daisuke S., Y, Mitsuhiro, I, Fitri , A, Yenni, Y, Ririka, Y, Shah , A, Silveira, H & Shigeto , Y 2019, 'Anopheline antiplatelet protein from mosquito saliva regulates blood feeding behavior' pp. 3129-3140. https://doi.org/10.1038/s41598-019-39960-2

Anopheline antiplatelet protein from mosquito saliva regulates blood feeding behavior. / Ashekul, Islam,; Talha Bin, Emran; Daisuke S., Yamamoto,; Mitsuhiro, Iyori,; Fitri , Amelia, ; Yenni, Yusuf,; Ririka, Yamaguchi, ; Shah , Alam,; Silveira, H; Shigeto , Yoshida,.

2019. 3129-3140.

Research output: Contribution to conferencePaper

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T1 - Anopheline antiplatelet protein from mosquito saliva regulates blood feeding behavior

AU - Ashekul, Islam,

AU - Talha Bin, Emran

AU - Daisuke S., Yamamoto,

AU - Mitsuhiro, Iyori,

AU - Fitri , Amelia,

AU - Yenni, Yusuf,

AU - Ririka, Yamaguchi,

AU - Shah , Alam,

AU - Silveira, H

AU - Shigeto , Yoshida,

PY - 2019/2/28

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N2 - The saliva of hematophagous arthropods is enriched with a complex mixture of antihemostatic molecules, the biological functions of which are largely unknown. Anopheline antiplatelet protein (AAPP) from malaria vector mosquito exhibits strong antiplatelet activity when bound directly to host collagen by its C-terminus and through its N-terminus with Ca2+-binding activity. To investigate the biological functions of AAPP in blood feeding behavior and malaria transmission, we generated transgenic Anopheles stephensi mosquito lines expressing anti-AAPP antibody single-chain fragment (scFv) in their salivary glands. The AAPP-specific collagen-binding activity was completely abolished by AAPP-scFv complex formation in the saliva. Probing and prediuresis time, feeding success, blood meal size, and fecundity, which are all fitness characteristics, were significantly reduced in the transgenic mosquitoes. However, oocysts number in these mosquitoes were not significantly reduced following blood meal intake from Plasmodium berghei-infected mice. These results show that although AAPP plays an important role in mosquito blood feeding, its neutralizing activity did not affect sporogonic development in our laboratory model, but its high fitness cost would pose a survival risk for parasite-infected mosquitoes in nature.

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