Anopheles aquasalis transcriptome reveals autophagic responses to Plasmodium vivax midgut invasion

Rosa Amélia Gonçalves Santana, Oliveira, Maurício Costa, Cabral, Iria, Junior, Rubens Celso Andrade Silva, de Sousa, Débora Raysa Teixeira, Lucas Silva Ferreira, Marcus V G Lacerda, Wuelton Marcelo Monteiro, P. Abrantes, Guerra, Maria Vinítius Graças Barbosa, H Silveira

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

BACKGROUND:
Elimination of malaria depends on mastering transmission and understanding the biological basis of Plasmodium infection in the vector. The first mosquito organ to interact with the parasite is the midgut and its transcriptomic characterization during infection can reveal effective antiplasmodial responses able to limit the survival of the parasite. The vector response to Plasmodium vivax is not fully characterized, and its specificities when compared with other malaria parasites can be of fundamental interest for specific control measures.
METHODS:
Experimental infections were performed using a membrane-feeding device. Three groups were used: P. vivax-blood-fed, blood-fed on inactivated gametocytes, and unfed mosquitoes. Twenty-four hours after feeding, the mosquitoes were dissected and the midgut collected for transcriptomic analysis using RNAseq. Nine cDNA libraries were generated and sequenced on an Illumina HiSeq2500. Readings were checked for quality control and analysed using the Trinity platform for de novo transcriptome assembly. Transcript quantification was performed and the transcriptome was functionally annotated. Differential expression gene analysis was carried out. The role of the identified mechanisms was further explored using functional approaches.
RESULTS:
Forty-nine genes were identified as being differentially expressed with P. vivax infection: 34 were upregulated and 15 were downregulated. Half of the P. vivax-related differentially expressed genes could be related to autophagy; therefore, the effect of the known inhibitor (wortmannin) and activator (spermidine) was tested on the infection outcome. Autophagic activation significantly reduced the intensity and prevalence of infection. This was associated with transcription alterations of the autophagy regulating genes Beclin, DRAM and Apg8.
CONCLUSIONS:
Our data indicate that P. vivax invasion of An. aquasalis midgut epithelium triggers an autophagic response and its activation reduces infection. This suggests a novel mechanism that mosquitoes can use to fight Plasmodium infection.
Original languageEnglish
Article number261
Pages (from-to)261-275
Number of pages14
JournalParasites & Vectors
Volume12
Issue number1
DOIs
Publication statusPublished - 24 May 2019

Keywords

  • Anopheles mosquitoes
  • Autophagy
  • Host parasite interactions
  • Malaria control
  • Malaria transmission
  • Plasmodium vivax

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

Fingerprint Dive into the research topics of 'Anopheles aquasalis transcriptome reveals autophagic responses to Plasmodium vivax midgut invasion'. Together they form a unique fingerprint.

  • Cite this

    Santana, R. A. G., Maurício Costa, O., Iria, C., Rubens Celso Andrade Silva, J., Débora Raysa Teixeira, D. S., Ferreira, L. S., Lacerda, M. V. G., Monteiro, W. M., Abrantes, P., Maria Vinítius Graças Barbosa, G., & Silveira, H. (2019). Anopheles aquasalis transcriptome reveals autophagic responses to Plasmodium vivax midgut invasion. Parasites & Vectors, 12(1), 261-275. [261]. https://doi.org/10.1186/s13071-019-3506-8