TY - JOUR
T1 - ANKH and susceptibility to and severity of ankylosing spondylitis
AU - Pimentel-Santos, Fernando Manuel
AU - Ligeiro, Dario
AU - Matos, Mafalda
AU - Mourão, Ana Filipa
AU - Vieira De Sousa, Elsa
AU - Pinto, Patricia
AU - Ribeiro, Ana
AU - Santos, Helena
AU - Barcelos, Anabela
AU - Godinho, Fatima
AU - Cruz, Margarida
AU - Fonseca, Joao Eurico
AU - Guedes-Pinto, Henrique
AU - Trindade, Helder
AU - Brown, Matthew A.
AU - Branco, Jaime C.
AU - De Matos, A. A.
AU - Ribeiro, C.
AU - Bravo Pimentão, J.
AU - Mateus, M.
AU - Nero, P.
AU - Araújo, P.
AU - Falcão, S.
AU - Pinto, T. L.
AU - Castelão, W.
AU - Caetano-Lopes, J.
AU - Silva, C.
AU - Simões, E.
AU - Madeira, H.
AU - Vaz Patto, J.
AU - Ferreira, J.
AU - Micaelo, M.
AU - Mediavilla, M. J.
AU - Sousa, M.
AU - Soares Branco, P.
AU - Canas Da Silva, J.
AU - Garcês, S.
AU - Tavares, V.
AU - Costa, J. A.
AU - Costa, L.
AU - Afonso, M. C.
AU - Bogas, M.
AU - Alcino, S.
AU - Silva, I.
AU - Ambrósio, C.
AU - Sequeira, G.
AU - Cravo, A. R.
AU - Santos, R. A.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Objective. Unconfirmed reports describe association of ankylosing spondylitis (AS) with several candidate genes including ANKH. Cellular export of inorganic pyrophosphate is regulated by the ANK protein, and mutant mice (ank/ank), which have a premature stop codon in the 3′ end of the ank gene, develop severe ankylosis. We tested the association between single-nucleotide polymorphisms (SNP) in these genes and susceptibility to AS in a population of patients with AS. We investigated the role of these genes in terms of functional (BASFI) and metrological (BASMI) measures, and the association with radiological severity (mSASSS). Methods. Our study was conducted on 355 patients with AS and 95 ethnically matched healthy controls. AS was defined according to the modified New York criteria. Four SNP in ANKH (rs27356, rs26307, rs25957, and rs28006) were genotyped. Association analysis was performed using Cochrane-Armitage and linear regression tests for dichotomous and quantitative variables. Analyses of Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), BASFI, and mSASSS were controlled for sex and disease duration. Results. None of the 4 markers showed significant single-locus disease associations (p > 0.05), suggesting that ANKH was not a major determinant of AS susceptibility in our population. No association was observed between these SNP and age at symptom onset, BASDAI, BASFI, BASMI, or mSASSS. Conclusion. These results confirm data in white Europeans that ANKH is probably not a major determinant of susceptibility to AS. ANKH polymorphisms do not markedly influence AS disease severity, as measured by BASMI and mSASSS. The Journal of Rheumatology
AB - Objective. Unconfirmed reports describe association of ankylosing spondylitis (AS) with several candidate genes including ANKH. Cellular export of inorganic pyrophosphate is regulated by the ANK protein, and mutant mice (ank/ank), which have a premature stop codon in the 3′ end of the ank gene, develop severe ankylosis. We tested the association between single-nucleotide polymorphisms (SNP) in these genes and susceptibility to AS in a population of patients with AS. We investigated the role of these genes in terms of functional (BASFI) and metrological (BASMI) measures, and the association with radiological severity (mSASSS). Methods. Our study was conducted on 355 patients with AS and 95 ethnically matched healthy controls. AS was defined according to the modified New York criteria. Four SNP in ANKH (rs27356, rs26307, rs25957, and rs28006) were genotyped. Association analysis was performed using Cochrane-Armitage and linear regression tests for dichotomous and quantitative variables. Analyses of Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), BASFI, and mSASSS were controlled for sex and disease duration. Results. None of the 4 markers showed significant single-locus disease associations (p > 0.05), suggesting that ANKH was not a major determinant of AS susceptibility in our population. No association was observed between these SNP and age at symptom onset, BASDAI, BASFI, BASMI, or mSASSS. Conclusion. These results confirm data in white Europeans that ANKH is probably not a major determinant of susceptibility to AS. ANKH polymorphisms do not markedly influence AS disease severity, as measured by BASMI and mSASSS. The Journal of Rheumatology
KW - Ankylosing spondylitis
KW - Genetic predisposition
KW - Morbidity
UR - http://www.scopus.com/inward/record.url?scp=84855415819&partnerID=8YFLogxK
U2 - 10.3899/jrheum.110681
DO - 10.3899/jrheum.110681
M3 - Article
C2 - 22089454
AN - SCOPUS:84855415819
SN - 0315-162X
VL - 39
SP - 131
EP - 134
JO - Journal of Rheumatology
JF - Journal of Rheumatology
IS - 1
ER -