Anion formation in gas-phase potassium-uridine collisions

Diogo Almeida, F. Ferreira da Silva, Janina Kopyra, Gustavo Garcia, Paulo Limão-Vieira

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study we report negative ion fragmentation patterns obtained from time-of-flight (TOF) mass spectrometry in potassium-uridine collisions at 30, 70 and 100 eV. The dominant fragments from uridine are also the most abundant from the individual units in potassium collisions with uracil, tetrahydrofuran (THF) and D-ribose. We observe no dehydrogenated parent anion formation. Special emphasis is given to the dissociation mechanisms lending support to the breaking of the N-glycosidic bond as an initial step in the fragmentation of the transient negative ion (TNI). Within their role as sugar unit surrogates, THF and D-ribose (DR) provide useful information for the study of uridine, and presumably other nucleosides from the point of view of DNA/RNA subunits integrity in the biological environment. (C) 2014 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)243-247
Number of pages5
JournalInternational Journal of Mass Spectrometry
Volume365-366
DOIs
Publication statusPublished - 15 May 2014

Fingerprint

ribose
Uridine
tetrahydrofuran
negative ions
Anions
Potassium
potassium
fragmentation
Negative ions
Ribose
Gases
vapor phases
anions
nucleosides
uracil
collisions
sugars
integrity
mass spectroscopy
Uracil

Keywords

  • Atomic collisions
  • Electron transfer
  • Negative ion
  • Nucleoside
  • Uridine

Cite this

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title = "Anion formation in gas-phase potassium-uridine collisions",
abstract = "In this study we report negative ion fragmentation patterns obtained from time-of-flight (TOF) mass spectrometry in potassium-uridine collisions at 30, 70 and 100 eV. The dominant fragments from uridine are also the most abundant from the individual units in potassium collisions with uracil, tetrahydrofuran (THF) and D-ribose. We observe no dehydrogenated parent anion formation. Special emphasis is given to the dissociation mechanisms lending support to the breaking of the N-glycosidic bond as an initial step in the fragmentation of the transient negative ion (TNI). Within their role as sugar unit surrogates, THF and D-ribose (DR) provide useful information for the study of uridine, and presumably other nucleosides from the point of view of DNA/RNA subunits integrity in the biological environment. (C) 2014 Elsevier B.V. All rights reserved.",
keywords = "Atomic collisions , Electron transfer , Negative ion , Nucleoside , Uridine",
author = "Diogo Almeida and {da Silva}, {F. Ferreira} and Janina Kopyra and Gustavo Garcia and Paulo Lim{\~a}o-Vieira",
note = "D.A. and F.F.S. acknowledge the Portuguese Foundation for Science and Technology (FCT-MEC) for post-doctoral SFRH/BPD/68979/2010 and SFRH/BPD/99261/2013 grants, respectively. P.L.V. acknowledges his visiting Professor position at The Open University, UK. We also acknowledge partial funding from PEst-OE/FIS/UI0068/2011 and PTDC/FIS-ATO/1832/2012 grants through FCT-MEC, and from the Spanish Ministerio de Economia y Competitividad (Project No. FIS 2012-31230). Some of this work forms part of the EU/ESF COST Action Nano-IBCT-MP1002. J.K. acknowledges support from EU/ESF COST Action MP1002 (Nano-IBCT) for a short term scientific visit to Lisbon.",
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month = "5",
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language = "English",
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journal = "International Journal of Mass Spectrometry",
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publisher = "Elsevier Science B.V., Amsterdam.",

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Anion formation in gas-phase potassium-uridine collisions. / Almeida, Diogo; da Silva, F. Ferreira; Kopyra, Janina; Garcia, Gustavo; Limão-Vieira, Paulo.

In: International Journal of Mass Spectrometry, Vol. 365-366, 15.05.2014, p. 243-247.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anion formation in gas-phase potassium-uridine collisions

AU - Almeida, Diogo

AU - da Silva, F. Ferreira

AU - Kopyra, Janina

AU - Garcia, Gustavo

AU - Limão-Vieira, Paulo

N1 - D.A. and F.F.S. acknowledge the Portuguese Foundation for Science and Technology (FCT-MEC) for post-doctoral SFRH/BPD/68979/2010 and SFRH/BPD/99261/2013 grants, respectively. P.L.V. acknowledges his visiting Professor position at The Open University, UK. We also acknowledge partial funding from PEst-OE/FIS/UI0068/2011 and PTDC/FIS-ATO/1832/2012 grants through FCT-MEC, and from the Spanish Ministerio de Economia y Competitividad (Project No. FIS 2012-31230). Some of this work forms part of the EU/ESF COST Action Nano-IBCT-MP1002. J.K. acknowledges support from EU/ESF COST Action MP1002 (Nano-IBCT) for a short term scientific visit to Lisbon.

PY - 2014/5/15

Y1 - 2014/5/15

N2 - In this study we report negative ion fragmentation patterns obtained from time-of-flight (TOF) mass spectrometry in potassium-uridine collisions at 30, 70 and 100 eV. The dominant fragments from uridine are also the most abundant from the individual units in potassium collisions with uracil, tetrahydrofuran (THF) and D-ribose. We observe no dehydrogenated parent anion formation. Special emphasis is given to the dissociation mechanisms lending support to the breaking of the N-glycosidic bond as an initial step in the fragmentation of the transient negative ion (TNI). Within their role as sugar unit surrogates, THF and D-ribose (DR) provide useful information for the study of uridine, and presumably other nucleosides from the point of view of DNA/RNA subunits integrity in the biological environment. (C) 2014 Elsevier B.V. All rights reserved.

AB - In this study we report negative ion fragmentation patterns obtained from time-of-flight (TOF) mass spectrometry in potassium-uridine collisions at 30, 70 and 100 eV. The dominant fragments from uridine are also the most abundant from the individual units in potassium collisions with uracil, tetrahydrofuran (THF) and D-ribose. We observe no dehydrogenated parent anion formation. Special emphasis is given to the dissociation mechanisms lending support to the breaking of the N-glycosidic bond as an initial step in the fragmentation of the transient negative ion (TNI). Within their role as sugar unit surrogates, THF and D-ribose (DR) provide useful information for the study of uridine, and presumably other nucleosides from the point of view of DNA/RNA subunits integrity in the biological environment. (C) 2014 Elsevier B.V. All rights reserved.

KW - Atomic collisions

KW - Electron transfer

KW - Negative ion

KW - Nucleoside

KW - Uridine

U2 - 10.1016/j.ijms.2014.01.023

DO - 10.1016/j.ijms.2014.01.023

M3 - Article

VL - 365-366

SP - 243

EP - 247

JO - International Journal of Mass Spectrometry

JF - International Journal of Mass Spectrometry

SN - 1387-3806

ER -