TY - JOUR
T1 - Angola’s 2013 dengue outbreak
T2 - Clinical, laboratory and molecular analyses of cases from four Portuguese institutions
AU - Parreira, Ricardo
AU - Conceição, Maria Cláudia Gomes dos Santos Rodrigues da
AU - Centeno-Lima, Sónia Chavarria Alves Ferreira Centeno
AU - Marques, Nuno
AU - Saraiva, José da Cunha
AU - Abreu, Cândida
AU - Sá, Laura
AU - Sarmento, António
AU - Atouguia, Jorge
AU - Moneti, Virgínia
AU - Azevedo, Telma
AU - Nina, Jaime
AU - Mansinho, Kamal
AU - Antunes, Abílio
AU - Teodósio, Rosa
AU - Nazareth, Teresa
AU - Seixas, Jorge
N1 - PMID:25212088
WOS:000343791000018
PY - 2014/9/1
Y1 - 2014/9/1
N2 - Introduction: Dengue virus (DENV) is the arbovirus with the widest impact on human health. In Africa in general, and in Angola in particular, the epidemiology and public health impact of DENV is far from clear. However, rapid population growth, unplanned urbanization, increased international travel, and the presence of virus major vector (Aedes aegypti) in the country suggest that DENV transmission may occur.Methodology: In parallel to the occurrence of a dengue outbreak affecting the capital of Angola, between March and July 2013 four Portuguese institutions diagnosed dengue infection in 146 individuals returning to Portugal. Clinical presentation, laboratory findings, and molecular analyses of partial viral genomic segments were performed.Results: The mean age of the individuals included in this study was 42 years old, the majority being men of Portuguese nationality, reporting various lengths of stay in Angola. Fever was the most reported clinical sign, being frequently associated (61.0%) with myalgia and headache. Hematological values, including hematocrit, white-blood cell and platelets counts, correlated with the absence of severe or complicated cases, or coagulation disorders. No deaths were observed. Viral NS1 was detected in 56.2% of the samples, and all NS1 negative cases had anti-dengue IgM antibodies. RT-PCR indicated the presence of DENV1, which was confirmed by phylogenetic analysis of 25 partial NS5 viral sequences.Conclusion: The DENV cases analyzed conformed to classical and uncomplicated dengue, caused by the suggested exclusive circulation of a genetically homogeneous DENV1 of genotype III, apparently with a single origin.
AB - Introduction: Dengue virus (DENV) is the arbovirus with the widest impact on human health. In Africa in general, and in Angola in particular, the epidemiology and public health impact of DENV is far from clear. However, rapid population growth, unplanned urbanization, increased international travel, and the presence of virus major vector (Aedes aegypti) in the country suggest that DENV transmission may occur.Methodology: In parallel to the occurrence of a dengue outbreak affecting the capital of Angola, between March and July 2013 four Portuguese institutions diagnosed dengue infection in 146 individuals returning to Portugal. Clinical presentation, laboratory findings, and molecular analyses of partial viral genomic segments were performed.Results: The mean age of the individuals included in this study was 42 years old, the majority being men of Portuguese nationality, reporting various lengths of stay in Angola. Fever was the most reported clinical sign, being frequently associated (61.0%) with myalgia and headache. Hematological values, including hematocrit, white-blood cell and platelets counts, correlated with the absence of severe or complicated cases, or coagulation disorders. No deaths were observed. Viral NS1 was detected in 56.2% of the samples, and all NS1 negative cases had anti-dengue IgM antibodies. RT-PCR indicated the presence of DENV1, which was confirmed by phylogenetic analysis of 25 partial NS5 viral sequences.Conclusion: The DENV cases analyzed conformed to classical and uncomplicated dengue, caused by the suggested exclusive circulation of a genetically homogeneous DENV1 of genotype III, apparently with a single origin.
KW - Dengue virus
KW - Imported viral diseases
KW - Laboratory surveillance
KW - Outbreaks
UR - http://www.scopus.com/inward/record.url?scp=84907014242&partnerID=8YFLogxK
U2 - 10.3855/jidc.4910
DO - 10.3855/jidc.4910
M3 - Article
C2 - 25212088
AN - SCOPUS:84907014242
SN - 2036-6590
VL - 8
SP - 1210
EP - 1215
JO - Journal Of Infection In Developing Countries
JF - Journal Of Infection In Developing Countries
IS - 9
ER -
