TY - JOUR
T1 - Analysis of carbohydrates in Lupinus albus stems on imposition of water deficit, using porous graphitic carbon liquid chromatography-electrospray ionization mass spectrometry
AU - Antonio, Carla
AU - Pinheiro, Carla
AU - Chaves, Maria Manuela
AU - Ricardo, Candido Pinto
AU - Ortuno, Maria Fernanda
AU - Thomas-Oates, Jane
PY - 2008/4/11
Y1 - 2008/4/11
N2 - This work reports the development and application of a negative ion mode online LC-ESI-MS method for studying the effect of water deficit on the carbohydrate content of Lupinus albus stems, using a porous graphitic carbon (PGC) stationary phase and an ion trap mass spectrometer. Using this method, separation and detection of several water soluble carbohydrates, ranging from mono-, di-, and oligosaccharides (raffinose, stachyose, and verbascose) to sugar alcohols was achieved in approximately 10 min. This on-line PGC-LC-ESI-MS method shows good linearity with correlation coefficients R-2 > 0.99, selectivity, short analysis time, and limits of detection (LOD) ranging from 0.4 to 9 pmol for sugars and 4-20 pmol for sugar alcohols. This PGC-LC-ESI-MS method is sensitive and allowed us to detect even small alterations in carbohydrate levels in L. albus stems that resulted from a mild/early water deficit (nmol g(-1) DW). This paper describes details of our method and its application to the quantitative analysis of water soluble underivatised carbohydrates extracted from L albus stem tissues that have been subjected to early and severe water deficit conditions, followed by a rewatering period. (c) 2008 Elsevier B.V. All rights reserved.
AB - This work reports the development and application of a negative ion mode online LC-ESI-MS method for studying the effect of water deficit on the carbohydrate content of Lupinus albus stems, using a porous graphitic carbon (PGC) stationary phase and an ion trap mass spectrometer. Using this method, separation and detection of several water soluble carbohydrates, ranging from mono-, di-, and oligosaccharides (raffinose, stachyose, and verbascose) to sugar alcohols was achieved in approximately 10 min. This on-line PGC-LC-ESI-MS method shows good linearity with correlation coefficients R-2 > 0.99, selectivity, short analysis time, and limits of detection (LOD) ranging from 0.4 to 9 pmol for sugars and 4-20 pmol for sugar alcohols. This PGC-LC-ESI-MS method is sensitive and allowed us to detect even small alterations in carbohydrate levels in L. albus stems that resulted from a mild/early water deficit (nmol g(-1) DW). This paper describes details of our method and its application to the quantitative analysis of water soluble underivatised carbohydrates extracted from L albus stem tissues that have been subjected to early and severe water deficit conditions, followed by a rewatering period. (c) 2008 Elsevier B.V. All rights reserved.
KW - carbohydrate
KW - water deficit
KW - early stress
KW - severe stress
KW - porous graphitic carbon
KW - mass spectrometry
KW - Lupinus albus
KW - stem
KW - ANION-EXCHANGE CHROMATOGRAPHY
KW - RAFFINOSE FAMILY OLIGOSACCHARIDES
KW - ARABIDOPSIS-THALIANA
KW - ION SUPPRESSION
KW - PERFORMANCE
KW - DROUGHT
KW - METABOLISM
KW - PLANT
KW - MECHANISMS
KW - TOLERANCE
UR - http://www.scopus.com/record/display.uri?eid=2-s2.0-40849135984&origin=inward&txGid=276cedb9d5af86f917e3cc63016cf616#
U2 - 10.1016/j.chroma.2008.02.010
DO - 10.1016/j.chroma.2008.02.010
M3 - Article
SN - 0021-9673
VL - 1187
SP - 111
EP - 118
JO - Journal Of Chromatography A
JF - Journal Of Chromatography A
IS - 1-2
ER -