Analysis of carbohydrates in Lupinus albus stems on imposition of water deficit, using porous graphitic carbon liquid chromatography-electrospray ionization mass spectrometry

Carla Antonio, Carla Pinheiro, Maria Manuela Chaves, Candido Pinto Ricardo, Maria Fernanda Ortuno, Jane Thomas-Oates

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

This work reports the development and application of a negative ion mode online LC-ESI-MS method for studying the effect of water deficit on the carbohydrate content of Lupinus albus stems, using a porous graphitic carbon (PGC) stationary phase and an ion trap mass spectrometer. Using this method, separation and detection of several water soluble carbohydrates, ranging from mono-, di-, and oligosaccharides (raffinose, stachyose, and verbascose) to sugar alcohols was achieved in approximately 10 min. This on-line PGC-LC-ESI-MS method shows good linearity with correlation coefficients R-2 > 0.99, selectivity, short analysis time, and limits of detection (LOD) ranging from 0.4 to 9 pmol for sugars and 4-20 pmol for sugar alcohols. This PGC-LC-ESI-MS method is sensitive and allowed us to detect even small alterations in carbohydrate levels in L. albus stems that resulted from a mild/early water deficit (nmol g(-1) DW). This paper describes details of our method and its application to the quantitative analysis of water soluble underivatised carbohydrates extracted from L albus stem tissues that have been subjected to early and severe water deficit conditions, followed by a rewatering period. (c) 2008 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalJournal Of Chromatography A
Volume1187
Issue number1-2
DOIs
Publication statusPublished - 11 Apr 2008

Keywords

  • carbohydrate
  • water deficit
  • early stress
  • severe stress
  • porous graphitic carbon
  • mass spectrometry
  • Lupinus albus
  • stem
  • ANION-EXCHANGE CHROMATOGRAPHY
  • RAFFINOSE FAMILY OLIGOSACCHARIDES
  • ARABIDOPSIS-THALIANA
  • ION SUPPRESSION
  • PERFORMANCE
  • DROUGHT
  • METABOLISM
  • PLANT
  • MECHANISMS
  • TOLERANCE

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