Analysis, design and engineering of simple iron-sulfur proteins: tales from rubredoxin and desulforedoxin

José J. G. Moura; Brian J. Goodfellow; Maria J. Romão; Frank Rusnak; Isabel Moura

doi:10.1080/02603599608032727

Analysis, design and engineering of simple iron-sulfur proteins: tales from rubredoxin and desulforedoxin

José J. G. Moura, Brian J. Goodfellow, Maria J. Romão, Frank Rusnak, Isabel Moura

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The most thoroughly characterized non-heme iron center in biology is Rubredoxin, the simplest member of the iron-sulfur class of metalloproteins. Rubredoxin contains a high-spin iron atom with tetrahedral coordination by four cysteinyl sulfur atoms. A structural variant of this center is found in Desulforedoxin, the smallest known Rubredoxin type protein. The 3D structure of both Rd and Dx has been determined at high resolution. These two proteins can therefore be used as case studies in which structural control by the polypeptide chain over the metal site can be discussed in detail.

Original language	English
Pages (from-to)	47-66
Number of pages	20
Journal	Comments On Inorganic Chemistry
Volume	19
Issue number	1
DOIs	https://doi.org/10.1080/02603599608032727
Publication status	Published - 1 Jan 1996

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title = "Analysis, design and engineering of simple iron-sulfur proteins: tales from rubredoxin and desulforedoxin",

abstract = "The most thoroughly characterized non-heme iron center in biology is Rubredoxin, the simplest member of the iron-sulfur class of metalloproteins. Rubredoxin contains a high-spin iron atom with tetrahedral coordination by four cysteinyl sulfur atoms. A structural variant of this center is found in Desulforedoxin, the smallest known Rubredoxin type protein. The 3D structure of both Rd and Dx has been determined at high resolution. These two proteins can therefore be used as case studies in which structural control by the polypeptide chain over the metal site can be discussed in detail.",

author = "Moura, {Jos{\'e} J. G.} and Goodfellow, {Brian J.} and Rom{\~a}o, {Maria J.} and Frank Rusnak and Isabel Moura",

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AU - Romão, Maria J.

AU - Rusnak, Frank

AU - Moura, Isabel

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N2 - The most thoroughly characterized non-heme iron center in biology is Rubredoxin, the simplest member of the iron-sulfur class of metalloproteins. Rubredoxin contains a high-spin iron atom with tetrahedral coordination by four cysteinyl sulfur atoms. A structural variant of this center is found in Desulforedoxin, the smallest known Rubredoxin type protein. The 3D structure of both Rd and Dx has been determined at high resolution. These two proteins can therefore be used as case studies in which structural control by the polypeptide chain over the metal site can be discussed in detail.

AB - The most thoroughly characterized non-heme iron center in biology is Rubredoxin, the simplest member of the iron-sulfur class of metalloproteins. Rubredoxin contains a high-spin iron atom with tetrahedral coordination by four cysteinyl sulfur atoms. A structural variant of this center is found in Desulforedoxin, the smallest known Rubredoxin type protein. The 3D structure of both Rd and Dx has been determined at high resolution. These two proteins can therefore be used as case studies in which structural control by the polypeptide chain over the metal site can be discussed in detail.

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Analysis, design and engineering of simple iron-sulfur proteins: tales from rubredoxin and desulforedoxin

Abstract

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