Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM

Ana Pimenta-Marques, Tania Perestrelo, Patricia Reis-Rodrigues, Paulo Duarte, Ana Ferreira-Silva, Mariana Lince-Faria, Mónica Bettencourt-Dias

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Abstract

Centrioles are part of centrosomes and cilia, which are microtubule organising centres (MTOC) with diverse functions. Despite their stability, centrioles can disappear during differentiation, such as in oocytes, but little is known about the regulation of their structural integrity. Our previous research revealed that the pericentriolar material (PCM) that surrounds centrioles and its recruiter, Polo kinase, are downregulated in oogenesis and sufficient for maintaining both centrosome structural integrity and MTOC activity. We now show that the expression of specific components of the centriole cartwheel and wall, including ANA1/CEP295, is essential for maintaining centrosome integrity. We find that Polo kinase requires ANA1 to promote centriole stability in cultured cells and eggs. In addition, ANA1 expression prevents the loss of centrioles observed upon PCM-downregulation. However, the centrioles maintained by overexpressing and tethering ANA1 are inactive, unlike the MTOCs observed upon tethering Polo kinase. These findings demonstrate that several centriole components are needed to maintain centrosome structure. Our study also highlights that centrioles are more dynamic than previously believed, with their structural stability relying on the continuous expression of multiple components.

Original languageEnglish
Pages (from-to)102-127
Number of pages26
JournalEmbo Reports
Volume25
Issue number1
DOIs
Publication statusPublished - 1 Jan 2024

Keywords

  • Centriole
  • Centrosome Integrity Maintenance
  • Cytoskeleton
  • Homeostasis
  • Oogenesis

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