Abstract
Anthocyanin lipophilization emerged as an efficient technique to improve their chemical stability, liposolubility and antioxidant properties for novel technological applications. This work describes an efficient method for the synthesis of cyanidin-3-glucoside-fatty acid conjugate using a Candida antarctica lipase B-rich extract, without further purification and retained in a porous membrane. Due to the enzyme retention within the membrane structure it was possible to improve the yield of the lipophilization reaction by 2.5-fold as well as obtaining the product in a shorter period of time comparing with its free form. Furthermore, the membrane retention allowed for enzyme reusability, since the same conversion yield was obtained in three consecutive reaction cycles.
| Original language | English |
|---|---|
| Article number | 125167 |
| Journal | Food Chemistry |
| Volume | 300 |
| DOIs | |
| Publication status | Published - 1 Dec 2019 |
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Keywords
- Anthocyanins lipophilization
- CalB-rich extract
- Enzyme immobilization
- Membrane process
- Naturally-occurring pigments
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An efficient method for anthocyanins lipophilization based on enzyme retention in membrane systems. / Guimarães, M.; Pérez-Gregorio, M.; Mateus, Nuno; de Freitas, Victor; Galinha, Cláudia F.; Crespo, João G.; Portugal, Carla A. M.; Cruz, Luís.
In: Food Chemistry, Vol. 300, 125167, 01.12.2019.Research output: Contribution to journal › Article
TY - JOUR
T1 - An efficient method for anthocyanins lipophilization based on enzyme retention in membrane systems
AU - Guimarães, M.
AU - Pérez-Gregorio, M.
AU - Mateus, Nuno
AU - de Freitas, Victor
AU - Galinha, Cláudia F.
AU - Crespo, João G.
AU - Portugal, Carla A. M.
AU - Cruz, Luís
N1 - info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F85293%2F2012/PT# PTDC/OCE-ETA/31250/2017. PT2020 (UID/QUI/50006/2019 -POCI/01/0145/FEDER/007265).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Anthocyanin lipophilization emerged as an efficient technique to improve their chemical stability, liposolubility and antioxidant properties for novel technological applications. This work describes an efficient method for the synthesis of cyanidin-3-glucoside-fatty acid conjugate using a Candida antarctica lipase B-rich extract, without further purification and retained in a porous membrane. Due to the enzyme retention within the membrane structure it was possible to improve the yield of the lipophilization reaction by 2.5-fold as well as obtaining the product in a shorter period of time comparing with its free form. Furthermore, the membrane retention allowed for enzyme reusability, since the same conversion yield was obtained in three consecutive reaction cycles.
AB - Anthocyanin lipophilization emerged as an efficient technique to improve their chemical stability, liposolubility and antioxidant properties for novel technological applications. This work describes an efficient method for the synthesis of cyanidin-3-glucoside-fatty acid conjugate using a Candida antarctica lipase B-rich extract, without further purification and retained in a porous membrane. Due to the enzyme retention within the membrane structure it was possible to improve the yield of the lipophilization reaction by 2.5-fold as well as obtaining the product in a shorter period of time comparing with its free form. Furthermore, the membrane retention allowed for enzyme reusability, since the same conversion yield was obtained in three consecutive reaction cycles.
KW - Anthocyanins lipophilization
KW - CalB-rich extract
KW - Enzyme immobilization
KW - Membrane process
KW - Naturally-occurring pigments
UR - http://www.scopus.com/inward/record.url?scp=85069043316&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2019.125167
DO - 10.1016/j.foodchem.2019.125167
M3 - Article
VL - 300
JO - Food Chemistry
JF - Food Chemistry
SN - 0308-8146
M1 - 125167
ER -