An efficient method for anthocyanins lipophilization based on enzyme retention in membrane systems

M. Guimarães, M. Pérez-Gregorio, Nuno Mateus, Victor de Freitas, Cláudia F. Galinha, João G. Crespo, Carla A. M. Portugal, Luís Cruz

Research output: Contribution to journalArticle

Abstract

Anthocyanin lipophilization emerged as an efficient technique to improve their chemical stability, liposolubility and antioxidant properties for novel technological applications. This work describes an efficient method for the synthesis of cyanidin-3-glucoside-fatty acid conjugate using a Candida antarctica lipase B-rich extract, without further purification and retained in a porous membrane. Due to the enzyme retention within the membrane structure it was possible to improve the yield of the lipophilization reaction by 2.5-fold as well as obtaining the product in a shorter period of time comparing with its free form. Furthermore, the membrane retention allowed for enzyme reusability, since the same conversion yield was obtained in three consecutive reaction cycles.

Original languageEnglish
Article number125167
JournalFood Chemistry
Volume300
DOIs
Publication statusPublished - 1 Dec 2019

Keywords

  • Anthocyanins lipophilization
  • CalB-rich extract
  • Enzyme immobilization
  • Membrane process
  • Naturally-occurring pigments

Fingerprint Dive into the research topics of 'An efficient method for anthocyanins lipophilization based on enzyme retention in membrane systems'. Together they form a unique fingerprint.

  • Cite this