Abstract

Amorphous/nanocrystalline silicon pi'ii'n devices fabricated on micromachined glass substrates are integrated with oligonucleotide-derivatized gold nanoparticles for a colorimetric detection method. The method enables the specific detection and quantification of unamplified nucleic acid sequences (DNA and RNA) without the need to functionalize the glass surface, allowing for resolution of single nucleotide differences between DNA and RNA sequences-single nucleotide polymorphism and mutation detection. The detector's substrate is glass and the sample is directly applied on the back side of the biosensor, ensuring a direct optical coupling of the assays with a concomitant maximum photon capture and the possibility to reuse the sensor. (c) 2007 American Institute of Physics.
Original languageEnglish
Article number023903
Pages (from-to)n/d
JournalApplied Physics Letters
Volume90
Issue number2
DOIs
Publication statusPublished - 1 Jan 2007

Fingerprint

Nucleic acid sequences
Nanocrystalline silicon
Amorphous silicon
Biosensors
Gold
Nanoparticles
Glass
Nucleotides
RNA
DNA
Substrates
Polymorphism
Oligonucleotides
Assays
Photons
Physics
Detectors
Sensors

Keywords

  • Bioassay
  • Biosensors
  • Colorimetry
  • DNA sequences
  • Gold
  • Mutagenesis
  • Nanostructured materials
  • Optical properties
  • Photons

Cite this

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title = "Amorphous/nanocrystalline silicon biosensor for the specific identification of unamplified nucleic acid sequences using gold nanoparticle probes",
abstract = "Amorphous/nanocrystalline silicon pi'ii'n devices fabricated on micromachined glass substrates are integrated with oligonucleotide-derivatized gold nanoparticles for a colorimetric detection method. The method enables the specific detection and quantification of unamplified nucleic acid sequences (DNA and RNA) without the need to functionalize the glass surface, allowing for resolution of single nucleotide differences between DNA and RNA sequences-single nucleotide polymorphism and mutation detection. The detector's substrate is glass and the sample is directly applied on the back side of the biosensor, ensuring a direct optical coupling of the assays with a concomitant maximum photon capture and the possibility to reuse the sensor. (c) 2007 American Institute of Physics.",
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AU - Doria, Gonçalo

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AU - Franco, Ricardo

AU - Fortunato, Elvira Maria Correia

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