Alpha-synuclein deregulates the expression of COL4A2 and impairs ER-Golgi function

Isabel Paiva, Gaurav Jain, Diana F. Lázaro, Kristina Gotovac Jerčić, Thomas Hentrich, Cemil Kerimoglu, Raquel Pinho, Èva M. Szegő, Susanne Burkhardt, Vincenzo Capece, Rashi Halder, Rezaul Islam, Mary Xylaki, Lucas A. Caldi Gomes, Anna Elisa Roser, Paul Lingor, Julia M. Schulze-Hentrich, Fran Borovečki, André Fischer, Tiago F. Outeiro

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Alpha-synuclein (aSyn) is the major protein component of Lewy bodies and Lewy neurites, the typical pathological hallmarks in Parkinson's disease (PD) and Dementia with Lewy bodies. aSyn is capable of inducing transcriptional deregulation, but the precise effect of specific aSyn mutants associated with familial forms of PD, remains unclear. Here, we used transgenic mice overexpressing human wild-type (WT) or A30P aSyn to compare the transcriptional profiles of the two animal models. We found that A30P aSyn promotes strong transcriptional deregulation and increases DNA binding. Interestingly, COL4A2, a major component of basement membranes, was found to be upregulated in both A30P aSyn transgenic mice and in dopaminergic neurons expressing A30P aSyn, suggesting a crucial role for collagen related genes in aSyn-induced toxicity. Finally, we observed that A30P aSyn alters Golgi morphology and increases the susceptibility to endoplasmic reticulum (ER) stress in dopaminergic cells. In total, our findings provide novel insight into the putative role of aSyn on transcription and on the molecular mechanisms involved, thereby opening novel avenues for future therapeutic interventions in PD and other synucleinopathies.

Original languageEnglish
Pages (from-to)121-135
Number of pages15
JournalNeurobiology of Disease
Volume119
DOIs
Publication statusPublished - 1 Nov 2018

Keywords

  • A30P alpha-synuclein
  • COL4A2
  • ER stress
  • Golgi fragmentation
  • Transcription deregulation

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