Algorithm to assess the presence of Aspergillus fumigatus resistant strains: the case of Norwegian sawmills

Carla Viegas, Beatriz Almeida, Liliana Aranha Caetano, Anani Afanou, Anne Straumfors, Cristina Veríssimo, Paulo Gonçalves, Raquel Sabino

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Association between selection pressure caused by the use of azole fungicides in sawmills and the development of fungal resistance has been described. The aim of this study was to implement an algorithm to assess the presence of Aspergillus section Fumigati resistant strains in sawmills. Eighty-six full-shift inhalable dust samples were collected from eleven industrial sawmills in Norway. Different culture media were used and molecular identification to species level in Aspergillus section Fumigati was done by calmodulin sequencing and TR34/L98H and TR46/Y121F/T289A mutations were screened by real-time PCR assay and confirmed by cyp51A sequencing. Six Fumigati isolates were identified as A. fumigatus sensu stricto and two of these grew on azole-supplemented media and were further analyzed by real-time PCR. One was confirmed to be a TR34/L98H mutant. The obtained results reinforce the need to assess the presence of A. fumigatus sensu stricto resistant isolates at other workplaces with fungicide pressure.

Original languageEnglish
JournalInternational Journal of Environmental Health Research
Publication statusAccepted/In press - 1 Jan 2020


  • Aspergillus fumigatus resistant isolates
  • cross-resistance
  • fungicide pressure
  • occupational exposure
  • Section Fumigati


Dive into the research topics of 'Algorithm to assess the presence of Aspergillus fumigatus resistant strains: the case of Norwegian sawmills'. Together they form a unique fingerprint.

Cite this