TY - JOUR
T1 - Alginate–Chitosan Membranes for the Encapsulation of Lavender Essential Oil and Development of Biomedical Applications Related to Wound Healing
AU - Cruz Sánchez, Encarnación
AU - García, María Teresa
AU - Pereira, Joana
AU - Oliveira, Filipe
AU - Craveiro, Rita
AU - Paiva, Alexandre
AU - Gracia, Ignacio
AU - García-Vargas, Jesús Manuel
AU - Duarte, Ana Rita C.
N1 - Funding Information:
info:eu-repo/grantAgreement/EC/H2020/725034/EU#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT#
info:eu-repo/grantAgreement/FCT/OE/2021.07780.BD/PT#
info:eu-repo/grantAgreement/FCT/OE/2022.13700.BD/PT#
This research was funded by the project Ref. SBPLY/17/180501/000311 from the Consejería de Educación, Cultura y Deportes of Junta de Comunidades de Castilla-La Mancha.
It has also been possible to carry out this work thanks to funding from the “Call for grants to carry out pre-doctoral stays in foreign Universities and Research Centres for UCLM doctoral students—Co-funded by the Collaboration Agreement between the University of Castilla-La Mancha and Banco Santander”.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/4/25
Y1 - 2023/4/25
N2 - Biopolymers such as chitosan (CHT) or alginate (ALG) are among the most prominent for health-related applications due to their broad bioactivity. Their combination for the preparation of membranes is hereby proposed as an application for wound healing with the incorporation of lavender essential oil (LEO), widely known for its antioxidant and antimicrobial properties. The preparation of CHT, CHT + LEO, ALG, ALG + LEO, and CHT/ALG + LEO membranes was accomplished, and its composition was analyzed using Fourier Transform Infrared Spectroscopy (FTIR). The water absorption capacity and oil release profile of the membranes revealed higher water uptake capacity when a lower LEO release was obtained. The combined CHT/ALG + LEO film showed a water uptake percentage of 638% after 48 h and a maximum LEO release concentration of 42 mg/L. Cytotoxicity and biocompatibility of the prepared membranes were studied using a HaCaT cell line, with an assessment of cell viability regarding film leachables, DNA quantification, and DAPI-phalloidin staining. The results revealed that the indirect contact of the prepared membranes via its leachables does not compromise cell viability, and upon direct contact, cells do not adhere or proliferate on the surface of the membranes. Moreover, the CHT/ALG + LEO membrane increases cell proliferation, making it suitable for applications in wound healing.
AB - Biopolymers such as chitosan (CHT) or alginate (ALG) are among the most prominent for health-related applications due to their broad bioactivity. Their combination for the preparation of membranes is hereby proposed as an application for wound healing with the incorporation of lavender essential oil (LEO), widely known for its antioxidant and antimicrobial properties. The preparation of CHT, CHT + LEO, ALG, ALG + LEO, and CHT/ALG + LEO membranes was accomplished, and its composition was analyzed using Fourier Transform Infrared Spectroscopy (FTIR). The water absorption capacity and oil release profile of the membranes revealed higher water uptake capacity when a lower LEO release was obtained. The combined CHT/ALG + LEO film showed a water uptake percentage of 638% after 48 h and a maximum LEO release concentration of 42 mg/L. Cytotoxicity and biocompatibility of the prepared membranes were studied using a HaCaT cell line, with an assessment of cell viability regarding film leachables, DNA quantification, and DAPI-phalloidin staining. The results revealed that the indirect contact of the prepared membranes via its leachables does not compromise cell viability, and upon direct contact, cells do not adhere or proliferate on the surface of the membranes. Moreover, the CHT/ALG + LEO membrane increases cell proliferation, making it suitable for applications in wound healing.
KW - alginate
KW - biocompatibility assessment
KW - cell viability
KW - chitosan
KW - lavender essential oil
KW - membranes
KW - water uptake ability
KW - wound healing
UR - http://www.scopus.com/inward/record.url?scp=85159264799&partnerID=8YFLogxK
U2 - 10.3390/molecules28093689
DO - 10.3390/molecules28093689
M3 - Article
C2 - 37175099
AN - SCOPUS:85159264799
SN - 1420-3049
VL - 28
JO - Molecules
JF - Molecules
IS - 9
M1 - 3689
ER -