Affinity tags in protein purification and peptide enrichment: An overview

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11 Citations (Scopus)


The reversible interaction between an affinity ligand and a complementary receptor has been widely explored in purification systems for several biomolecules. The development of tailored affinity ligands highly specific toward particular target biomolecules is one of the options in affinity purification systems. However, both genetic and chemical modifications in proteins and peptides widen the application of affinity ligand-tag receptors pairs toward universal capture and purification strategies. In particular, this chapter will focus on two case studies highly relevant for biotechnology and biomedical areas, namely the affinity tags and receptors employed on the production of recombinant fusion proteins, and the chemical modification of phosphate groups on proteins and peptides and the subsequent specific capture and enrichment, a mandatory step before further proteomic analysis.

Original languageEnglish
Title of host publicationProtein Downstream Processing
EditorsN. E. Labrou
Place of PublicationNew York
PublisherHumana Press
Number of pages26
ISBN (Electronic)978-1-62703-977-2
ISBN (Print)978-1-62703-976-5
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
PublisherHumana Press Inc.
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Affinity purification
  • Affinity tags
  • Fusion proteins
  • Phosphoproteomics
  • Recombinant proteins


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