@inbook{336d2a17b22743f7a20d4eee35ec5e1a,
title = "Affinity tags in protein purification and peptide enrichment: An overview",
abstract = "The reversible interaction between an affinity ligand and a complementary receptor has been widely explored in purification systems for several biomolecules. The development of tailored affinity ligands highly specific toward particular target biomolecules is one of the options in affinity purification systems. However, both genetic and chemical modifications in proteins and peptides widen the application of affinity ligand-tag receptors pairs toward universal capture and purification strategies. In particular, this chapter will focus on two case studies highly relevant for biotechnology and biomedical areas, namely the affinity tags and receptors employed on the production of recombinant fusion proteins, and the chemical modification of phosphate groups on proteins and peptides and the subsequent specific capture and enrichment, a mandatory step before further proteomic analysis.",
keywords = "Affinity purification, Affinity tags, Fusion proteins, Phosphoproteomics, Recombinant proteins",
author = "Pina, {Ana Sofia} and Batalha, {{\'I}ris L.} and Dias, {Ana M. G. C.} and Roque, {Ana Cec{\'i}lia A.}",
note = "info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F97585%2F2013/PT# info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F64427%2F2009/PT# FCT/MEC (UID/Multi/04378/ 2019); co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). A.M.G.C.D. for Junior Research contract under Sea2See project financed by FCT (PTDC/BII-BIO/28878/2017) and co-financed by ERDF under the PT2020 Partnership Agreement (LISBOA-01-0145-FEDER-028878).",
year = "2021",
doi = "10.1007/978-1-0716-0775-6_10",
language = "English",
isbn = "978-1-62703-976-5",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "107--132",
editor = "Labrou, {N. E.}",
booktitle = "Protein Downstream Processing",
address = "United States",
}