Affinity tags in protein purification and peptide enrichment: An overview

Ana Sofia Pina, Iris L Batalha, Ana Cecília A Roque

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

The reversible interaction between an affinity ligand and a complementary receptor has been widely explored in purification systems for several biomolecules. The development of tailored affinity ligands highly specific towards particular target biomolecules is one of the options in affinity purification systems. However, both genetic and chemical modifications on proteins and peptides widen the application of affinity ligand-tag receptor pairs towards universal capture and purification strategies. In particular, this chapter will focus on two case studies highly relevant for biotechnology and biomedical areas, namely, the affinity tags and receptors employed on the production of recombinant fusion proteins and the chemical modification of phosphate groups on proteins and peptides and the subsequent specific capture and enrichment, a mandatory step before further proteomic analysis.

Original languageEnglish
Title of host publicationProtein Downstream Processing
Subtitle of host publicationDesign, Development and Application of High and Low-Resolution Methods
EditorsNikolaos E. Labrou
PublisherHumana Press Inc.
Pages147-168
Number of pages22
ISBN (Electronic)978-1-62703-977-2
ISBN (Print)978-1-62703-976-5
DOIs
Publication statusPublished - 17 Feb 2014

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1129
ISSN (Print)1064-3745

Fingerprint

Ligands
Peptides
Recombinant Fusion Proteins
Proteins
Biotechnology
Proteomics
Phosphates

Keywords

  • Affinity Labels
  • Amino Acid Sequence
  • Molecular Sequence Data
  • Peptides
  • Proteins
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Review

Cite this

Pina, A. S., Batalha, I. L., & Roque, A. C. A. (2014). Affinity tags in protein purification and peptide enrichment: An overview. In N. E. Labrou (Ed.), Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods (pp. 147-168). (Methods in Molecular Biology; Vol. 1129). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-977-2_14
Pina, Ana Sofia ; Batalha, Iris L ; Roque, Ana Cecília A. / Affinity tags in protein purification and peptide enrichment : An overview. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods. editor / Nikolaos E. Labrou. Humana Press Inc., 2014. pp. 147-168 (Methods in Molecular Biology).
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Pina, AS, Batalha, IL & Roque, ACA 2014, Affinity tags in protein purification and peptide enrichment: An overview. in N E. Labrou (ed.), Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods. Methods in Molecular Biology, vol. 1129, Humana Press Inc., pp. 147-168. https://doi.org/10.1007/978-1-62703-977-2_14

Affinity tags in protein purification and peptide enrichment : An overview. / Pina, Ana Sofia; Batalha, Iris L; Roque, Ana Cecília A.

Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods. ed. / Nikolaos E. Labrou. Humana Press Inc., 2014. p. 147-168 (Methods in Molecular Biology; Vol. 1129).

Research output: Chapter in Book/Report/Conference proceedingChapter

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AB - The reversible interaction between an affinity ligand and a complementary receptor has been widely explored in purification systems for several biomolecules. The development of tailored affinity ligands highly specific towards particular target biomolecules is one of the options in affinity purification systems. However, both genetic and chemical modifications on proteins and peptides widen the application of affinity ligand-tag receptor pairs towards universal capture and purification strategies. In particular, this chapter will focus on two case studies highly relevant for biotechnology and biomedical areas, namely, the affinity tags and receptors employed on the production of recombinant fusion proteins and the chemical modification of phosphate groups on proteins and peptides and the subsequent specific capture and enrichment, a mandatory step before further proteomic analysis.

KW - Affinity Labels

KW - Amino Acid Sequence

KW - Molecular Sequence Data

KW - Peptides

KW - Proteins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Review

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Pina AS, Batalha IL, Roque ACA. Affinity tags in protein purification and peptide enrichment: An overview. In E. Labrou N, editor, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods. Humana Press Inc. 2014. p. 147-168. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-977-2_14