Advances in Lentivirus Purification

Ana Sofia Moreira, David Guia Cavaco, Tiago Q. Faria, Paula M. Alves, Manuel J. T. Carrondo, Cristina Peixoto

Research output: Contribution to journalReview articlepeer-review

42 Citations (Scopus)

Abstract

Lentiviral vectors (LVs) have been increasingly used as a tool for gene and cell therapies since they can stably integrate the genome in dividing and nondividing cells. LV production and purification processes have evolved substantially over the last decades. However, the increasing demands for higher quantities with more restrictive purity requirements are stimulating the development of novel materials and strategies to supply the market with LV in a cost-effective manner. A detailed review of each downstream process unit operation is performed, limitations, strengths, and potential outcomes being covered. Currently, the majority of large-scale LV manufacturing processes are still based on adherent cell culture, although it is known that the industry is migrating fast to suspension cultures. Regarding the purification strategy, it consists of batch chromatography and membrane technology. Nevertheless, new solutions are being created to improve the current production schemes and expand its clinical use.

Original languageEnglish
Article number2000019
JournalBiotechnology Journal
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 2021

Keywords

  • bioseparation
  • chromatography
  • downstream processing
  • lentiviral vectors

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