This invention relates to the process of obtaining a culture of adult human neural progenitor/stem cells obtained from epithelium and olfactory lamina propria of a human adult. Adult human neural stem/progenitor cells were tested using 26 biopsies of both female and male human, with ages ranging from 21 to 45 years old. This invention further relates to a serum-free culture medium formulation that enables the proliferation of adult human neural progenitor/stem cells for seven months, under strict serum-free conditions, with a doubling time of 15 days. This invention also provides a new culturing process for the proliferation of adult human neural progenitor/stem cells which obviate the formation of neurospheres. This invention provides a method of differentiating adult human neural progenitor/stem cells into immature neurons.; This invention shows that olfactory epithelium human cells are a reliable source for the recovery of spinal cord tissue injuries.
|IPC||C12N 5/ 0797 A I|
|Publication status||Published - 22 Feb 2007|