Adaptive DO-based control of substrate feeding in high cell density cultures operated under oxygen transfer limitation

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Abstract

The carbon source feeding strategy is crucial for the productivity of many biochemical processes. In high density and shear sensitive cultures the feed of the carbon source is frequently constrained by the bioreactor maximum oxygen transfer capacity. In order to maximise the product formation, these processes should be operated at low dissolved oxygen (DO) concentrations close to the limitation level. This operating strategy may be realised with a closed-loop controller that regulates the DO concentration through the manipulation of the carbon source feed rate. The performance of this controller may have a significant influence on the final product production and should be as accurate as possible. In this work we study the application of adaptive control for solving this problem focusing not only on stability but also on accuracy. Whenever possible the convergence trajectories to the set point are characterised mathematically. Concerning the instrumentation, two situations are covered i) only the DO Tension (DOT) is measured, ii) both DOT and off-gas composition are measured on-line. The controllers are tested in a pilot plant recombinant Pichia pastoris process.
Original languageEnglish
Title of host publicationAdaptive and Natural Computing Algorithms
Subtitle of host publicationProceedings of the International Conference in Coimbra, Portugal, 2005
EditorsBernardete Ribeiro, R. F. Albrecht, Andrej Dobnikar, David W. Pearson, Nigel C. Steele
PublisherSpringer
Pages300-303
Number of pages4
ISBN (Electronic)978-3-211-27389-0
ISBN (Print)978-3-211-24934-5
DOIs
Publication statusPublished - 1 Jan 2005
Event7th International Conference on Adaptive and Natural Computing Algorithms (ICANNGA) - Coimbra, Portugal
Duration: 21 Mar 200523 Mar 2005

Conference

Conference7th International Conference on Adaptive and Natural Computing Algorithms (ICANNGA)
CountryPortugal
CityCoimbra
Period21/03/0523/03/05

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