TY - JOUR
T1 - Adaptive divergence in wine yeasts and their wild relatives suggests a prominent role for introgressions and rapid evolution at noncoding sites
AU - Almeida, Pedro
AU - Barbosa, Raquel
AU - Bensasson, Douda
AU - Gonçalves, Paula
AU - Sampaio, José Paulo
N1 - Sem PDF.
Fundacao para a Ciencia e a Tecnologia, Portugal (PTDC/BIA-EVF/118618/2010;
PTDC/AGR-ALI/118590/2010; UID/Multi/04378/2013; SFRH/BD/77390/2011)
PY - 2017/4
Y1 - 2017/4
N2 - In Saccharomyces cerevisiae, the main yeast in wine fermentation, the opportunity to examine divergence at the molecular level between a domesticated lineage and its wild counterpart arose recently due to the identification of the closest relatives of wine strains, a wild population associated with Mediterranean oaks. As genomic data are available for a considerable number of representatives belonging to both groups, we used population genomics to estimate the degree and distribution of nucleotide variation between wine yeasts and their closest wild relatives. We found widespread genomewide divergence, particularly at noncoding sites, which, together with above average divergence in trans-acting DNA binding proteins, may suggest an important role for divergence at the level of transcriptional regulation. Nine outlier regions putatively under strong divergent selection were highlighted by a genomewide scan under stringent conditions. Several cases of introgressions, originating in the sibling species Saccharomyces paradoxus, were also identified in the Mediterranean oak population. FZF1 and SSU1, mostly known for conferring sulphite resistance in wine yeasts, were among the introgressed genes, although not fixed. Because the introgressions detected in our study are not found in wine strains, we hypothesize that ongoing divergent ecological selection segregates the two forms between the different niches. Together, our results provide a first insight into the extent and kind of divergence between wine yeasts and their closest wild relatives.
AB - In Saccharomyces cerevisiae, the main yeast in wine fermentation, the opportunity to examine divergence at the molecular level between a domesticated lineage and its wild counterpart arose recently due to the identification of the closest relatives of wine strains, a wild population associated with Mediterranean oaks. As genomic data are available for a considerable number of representatives belonging to both groups, we used population genomics to estimate the degree and distribution of nucleotide variation between wine yeasts and their closest wild relatives. We found widespread genomewide divergence, particularly at noncoding sites, which, together with above average divergence in trans-acting DNA binding proteins, may suggest an important role for divergence at the level of transcriptional regulation. Nine outlier regions putatively under strong divergent selection were highlighted by a genomewide scan under stringent conditions. Several cases of introgressions, originating in the sibling species Saccharomyces paradoxus, were also identified in the Mediterranean oak population. FZF1 and SSU1, mostly known for conferring sulphite resistance in wine yeasts, were among the introgressed genes, although not fixed. Because the introgressions detected in our study are not found in wine strains, we hypothesize that ongoing divergent ecological selection segregates the two forms between the different niches. Together, our results provide a first insight into the extent and kind of divergence between wine yeasts and their closest wild relatives.
KW - comparative population genomics
KW - divergent selection
KW - introgression
KW - microbe adaptive evolution
KW - Saccharomyces cerevisiae
KW - wine yeast domestication
UR - http://www.scopus.com/inward/record.url?scp=85016574659&partnerID=8YFLogxK
U2 - 10.1111/mec.14071
DO - 10.1111/mec.14071
M3 - Article
C2 - 28231394
AN - SCOPUS:85016574659
SN - 0962-1083
VL - 26
SP - 2167
EP - 2182
JO - Molecular Ecology
JF - Molecular Ecology
IS - 7
ER -