TY - JOUR
T1 - Adaptable stirred-tank culture strategies for large scale production of multicellular spheroid-based tumor cell models
AU - Santo, Vítor E.
AU - Estrada, Marta Falcão
AU - Rebelo, Sofia P.
AU - Abreu, Sofia
AU - Silva, Inês
AU - Pinto, Catarina
AU - Veloso, Susana C.
AU - Serra, Ana Teresa
AU - Boghaert, Erwin
AU - Alves, Paula M.
AU - Brito, Catarina
PY - 2016/3/10
Y1 - 2016/3/10
N2 - Currently there is an effort toward the development of in vitro cancer models more predictive of clinical efficacy. The onset of advanced analytical tools and imaging technologies has increased the utilization of spheroids in the implementation of high throughput approaches in drug discovery. Agitation-based culture systems are commonly proposed as an alternative method for the production of tumor spheroids, despite the scarce experimental evidence found in the literature. In this study, we demonstrate the robustness and reliability of stirred-tank cultures for the scalable generation of 3D cancer models. We developed standardized protocols to a panel of tumor cell lines from different pathologies and attained efficient tumor cell aggregation by tuning hydrodynamic parameters. Large numbers of spheroids were obtained (typically 1000-1500 spheroids/mL) presenting features of native tumors, namely morphology, proliferation and hypoxia gradients, in a cell line-dependent mode. Heterotypic 3D cancer models, based on co-cultures of tumor cells and fibroblasts, were also established in the absence or presence of additional physical support from an alginate matrix, with maintenance of high cell viability. Altogether, we demonstrate that 3D tumor cell model production in stirred-tank culture systems is a robust and versatile approach, providing reproducible tools for drug screening and target verification in pre-clinical oncology research.
AB - Currently there is an effort toward the development of in vitro cancer models more predictive of clinical efficacy. The onset of advanced analytical tools and imaging technologies has increased the utilization of spheroids in the implementation of high throughput approaches in drug discovery. Agitation-based culture systems are commonly proposed as an alternative method for the production of tumor spheroids, despite the scarce experimental evidence found in the literature. In this study, we demonstrate the robustness and reliability of stirred-tank cultures for the scalable generation of 3D cancer models. We developed standardized protocols to a panel of tumor cell lines from different pathologies and attained efficient tumor cell aggregation by tuning hydrodynamic parameters. Large numbers of spheroids were obtained (typically 1000-1500 spheroids/mL) presenting features of native tumors, namely morphology, proliferation and hypoxia gradients, in a cell line-dependent mode. Heterotypic 3D cancer models, based on co-cultures of tumor cells and fibroblasts, were also established in the absence or presence of additional physical support from an alginate matrix, with maintenance of high cell viability. Altogether, we demonstrate that 3D tumor cell model production in stirred-tank culture systems is a robust and versatile approach, providing reproducible tools for drug screening and target verification in pre-clinical oncology research.
KW - 3D cell culture
KW - Drug discovery
KW - In vitro cancer models
KW - Stirred-tank culture systems
KW - Tumor spheroids
UR - http://www.scopus.com/inward/record.url?scp=84957944330&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2016.01.031
DO - 10.1016/j.jbiotec.2016.01.031
M3 - Article
AN - SCOPUS:84957944330
SN - 0168-1656
VL - 221
SP - 118
EP - 129
JO - Journal of Biotechnology
JF - Journal of Biotechnology
ER -