Acute exercise amplifies inflammation in obese patients with COPD

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Abstract

Systemic inflammation has been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD) systemic effects. However, most COPD patients do not suffer from persistent systemic inflammation even after exacerbations and exercise and scientific evidence has provided conflicting results. Our aim is to evaluate inflammatory gene expression at rest and at 1 and 24 h after strenuous exercise in COPD patients and study the patient variables associated with inflammatory expression.

A cross-sectional study was conducted in COPD patients who were recruited on entry to a pulmonary rehabilitation (PR) program. Demographic, clinical and functional data were collected. Blood samples were collected and gene expression was analyzed by reverse transcriptase polymerase chain reaction for IFNg, ILIb, IL6, IL8, TNFa, TGFb1 and iNOS.

The study included 21 patients (15 men, 71.4%), mean age 66.1 years old (SD = 8.27), mean FEV1 46.76% (SD 20.90%), 67% belonging to GOLD grade D, mean BODE index of 3.9, 90.5% with smoking history, mean BMI 25.81 (SD=4.87), median of 1.29 exacerbations in the previous year.

There was no statistical significant difference between inflammatory expression at rest and at 1 h and 24 h after the maximal exercise test for all tested genes.

We found an association between BMI and inflammatory expression at all the points of time checked, a slight inverse association occurs with low BMI for mRNA IL1b, IL6, TNFa, TGFb1 and iNOS, and there was a more pronounced positive association for obese patients for all tested genes.

This preliminary study did not show an enhanced inflammatory gene expression from rest to 1 h and 24 h after short-term exercise, but did show an increased inflammatory gene expression in both BMI extremes, both at rest and after exercise, suggesting not only malnourishment, but also obesity as potential links between COPD and systemic inflammation. Studies with larger samples and designed to definitely exclude OSA or OHS as confounding factors in obese patients are required. (C) 2016 Sociedade Portuguesa de Pneumologia. Published by Elsevier Espana, S.L.U. This is an open access article under the CC BY-NC-ND license.

Original languageEnglish
Pages (from-to)315-322
Number of pages8
JournalRevista Portuguesa de Pneumologia
Volume22
Issue number6
DOIs
Publication statusPublished - 2016

Keywords

  • COPD
  • Inflammation
  • Exercise
  • Obesity
  • Reverse transcriptase
  • polymerase chain
  • reaction
  • OBSTRUCTIVE PULMONARY-DISEASE
  • SYSTEMIC INFLAMMATION
  • PLASMA-LEVELS
  • TNF-ALPHA
  • COMORBIDITIES
  • VALIDATION
  • MUSCLE
  • IL-1-BETA
  • DYSPNEA
  • CELLS

Cite this

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title = "Acute exercise amplifies inflammation in obese patients with COPD",
abstract = "Systemic inflammation has been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD) systemic effects. However, most COPD patients do not suffer from persistent systemic inflammation even after exacerbations and exercise and scientific evidence has provided conflicting results. Our aim is to evaluate inflammatory gene expression at rest and at 1 and 24 h after strenuous exercise in COPD patients and study the patient variables associated with inflammatory expression.A cross-sectional study was conducted in COPD patients who were recruited on entry to a pulmonary rehabilitation (PR) program. Demographic, clinical and functional data were collected. Blood samples were collected and gene expression was analyzed by reverse transcriptase polymerase chain reaction for IFNg, ILIb, IL6, IL8, TNFa, TGFb1 and iNOS.The study included 21 patients (15 men, 71.4{\%}), mean age 66.1 years old (SD = 8.27), mean FEV1 46.76{\%} (SD 20.90{\%}), 67{\%} belonging to GOLD grade D, mean BODE index of 3.9, 90.5{\%} with smoking history, mean BMI 25.81 (SD=4.87), median of 1.29 exacerbations in the previous year.There was no statistical significant difference between inflammatory expression at rest and at 1 h and 24 h after the maximal exercise test for all tested genes.We found an association between BMI and inflammatory expression at all the points of time checked, a slight inverse association occurs with low BMI for mRNA IL1b, IL6, TNFa, TGFb1 and iNOS, and there was a more pronounced positive association for obese patients for all tested genes.This preliminary study did not show an enhanced inflammatory gene expression from rest to 1 h and 24 h after short-term exercise, but did show an increased inflammatory gene expression in both BMI extremes, both at rest and after exercise, suggesting not only malnourishment, but also obesity as potential links between COPD and systemic inflammation. Studies with larger samples and designed to definitely exclude OSA or OHS as confounding factors in obese patients are required. (C) 2016 Sociedade Portuguesa de Pneumologia. Published by Elsevier Espana, S.L.U. This is an open access article under the CC BY-NC-ND license.",
keywords = "COPD, Inflammation, Exercise, Obesity, Reverse transcriptase, polymerase chain, reaction, OBSTRUCTIVE PULMONARY-DISEASE, SYSTEMIC INFLAMMATION, PLASMA-LEVELS, TNF-ALPHA, COMORBIDITIES, VALIDATION, MUSCLE, IL-1-BETA, DYSPNEA, CELLS",
author = "F. Rodrigues and Papoila, {A. L.} and Gomes, {Maria Jo{\~a}o Costa Santos Mattos Marques} and D. Ligeiro and Trindade, {Helder Fernando Branco}",
year = "2016",
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journal = "Revista Portuguesa de Pneumologia",
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TY - JOUR

T1 - Acute exercise amplifies inflammation in obese patients with COPD

AU - Rodrigues, F.

AU - Papoila, A. L.

AU - Gomes, Maria João Costa Santos Mattos Marques

AU - Ligeiro, D.

AU - Trindade, Helder Fernando Branco

PY - 2016

Y1 - 2016

N2 - Systemic inflammation has been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD) systemic effects. However, most COPD patients do not suffer from persistent systemic inflammation even after exacerbations and exercise and scientific evidence has provided conflicting results. Our aim is to evaluate inflammatory gene expression at rest and at 1 and 24 h after strenuous exercise in COPD patients and study the patient variables associated with inflammatory expression.A cross-sectional study was conducted in COPD patients who were recruited on entry to a pulmonary rehabilitation (PR) program. Demographic, clinical and functional data were collected. Blood samples were collected and gene expression was analyzed by reverse transcriptase polymerase chain reaction for IFNg, ILIb, IL6, IL8, TNFa, TGFb1 and iNOS.The study included 21 patients (15 men, 71.4%), mean age 66.1 years old (SD = 8.27), mean FEV1 46.76% (SD 20.90%), 67% belonging to GOLD grade D, mean BODE index of 3.9, 90.5% with smoking history, mean BMI 25.81 (SD=4.87), median of 1.29 exacerbations in the previous year.There was no statistical significant difference between inflammatory expression at rest and at 1 h and 24 h after the maximal exercise test for all tested genes.We found an association between BMI and inflammatory expression at all the points of time checked, a slight inverse association occurs with low BMI for mRNA IL1b, IL6, TNFa, TGFb1 and iNOS, and there was a more pronounced positive association for obese patients for all tested genes.This preliminary study did not show an enhanced inflammatory gene expression from rest to 1 h and 24 h after short-term exercise, but did show an increased inflammatory gene expression in both BMI extremes, both at rest and after exercise, suggesting not only malnourishment, but also obesity as potential links between COPD and systemic inflammation. Studies with larger samples and designed to definitely exclude OSA or OHS as confounding factors in obese patients are required. (C) 2016 Sociedade Portuguesa de Pneumologia. Published by Elsevier Espana, S.L.U. This is an open access article under the CC BY-NC-ND license.

AB - Systemic inflammation has been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD) systemic effects. However, most COPD patients do not suffer from persistent systemic inflammation even after exacerbations and exercise and scientific evidence has provided conflicting results. Our aim is to evaluate inflammatory gene expression at rest and at 1 and 24 h after strenuous exercise in COPD patients and study the patient variables associated with inflammatory expression.A cross-sectional study was conducted in COPD patients who were recruited on entry to a pulmonary rehabilitation (PR) program. Demographic, clinical and functional data were collected. Blood samples were collected and gene expression was analyzed by reverse transcriptase polymerase chain reaction for IFNg, ILIb, IL6, IL8, TNFa, TGFb1 and iNOS.The study included 21 patients (15 men, 71.4%), mean age 66.1 years old (SD = 8.27), mean FEV1 46.76% (SD 20.90%), 67% belonging to GOLD grade D, mean BODE index of 3.9, 90.5% with smoking history, mean BMI 25.81 (SD=4.87), median of 1.29 exacerbations in the previous year.There was no statistical significant difference between inflammatory expression at rest and at 1 h and 24 h after the maximal exercise test for all tested genes.We found an association between BMI and inflammatory expression at all the points of time checked, a slight inverse association occurs with low BMI for mRNA IL1b, IL6, TNFa, TGFb1 and iNOS, and there was a more pronounced positive association for obese patients for all tested genes.This preliminary study did not show an enhanced inflammatory gene expression from rest to 1 h and 24 h after short-term exercise, but did show an increased inflammatory gene expression in both BMI extremes, both at rest and after exercise, suggesting not only malnourishment, but also obesity as potential links between COPD and systemic inflammation. Studies with larger samples and designed to definitely exclude OSA or OHS as confounding factors in obese patients are required. (C) 2016 Sociedade Portuguesa de Pneumologia. Published by Elsevier Espana, S.L.U. This is an open access article under the CC BY-NC-ND license.

KW - COPD

KW - Inflammation

KW - Exercise

KW - Obesity

KW - Reverse transcriptase

KW - polymerase chain

KW - reaction

KW - OBSTRUCTIVE PULMONARY-DISEASE

KW - SYSTEMIC INFLAMMATION

KW - PLASMA-LEVELS

KW - TNF-ALPHA

KW - COMORBIDITIES

KW - VALIDATION

KW - MUSCLE

KW - IL-1-BETA

KW - DYSPNEA

KW - CELLS

U2 - 10.1016/j.rppnen.2016.05.005

DO - 10.1016/j.rppnen.2016.05.005

M3 - Article

VL - 22

SP - 315

EP - 322

JO - Revista Portuguesa de Pneumologia

JF - Revista Portuguesa de Pneumologia

SN - 0873-2159

IS - 6

ER -