A unique glyceryl diglycoside identified in the thermophilic, radiation-resistant bacterium Rubrobacter xylanophilus

Pedro Lamosa, Eva C. Lourenço, Filipa d’Avó, Ana Nobre, Tiago M. Bandeiras, Milton S. da Costa, Maria Rita Lima, Maria Helena Santos

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The solute pool of the actinobacterium Rubrobacter xylanophilus has been investigated as a function of the growth temperature and concentration of NaCl in the medium (Empadinhas et al. Extremophiles 11: 667–673, 2007). Changing the carbon source from glucose to maltose in a minimal growth medium led to the accumulation of an unknown organic compound whose structure was investigated by NMR and confirmed by chemical synthesis in the present study as: (2R)-2-(1-O-α-d-mannopyranosyl)-3-(1-O-α-d-glucopyranosyl)-d-glycerate (MGlyG). In addition to this newly identified diglycoside, the solute pool of R. xylanophilus included trehalose, mannosylglycerate, di-myo-inositol phosphate and di-N-acetyl-glucosamine phosphate. The structure of MGlyG was established by NMR and confirmed by chemical synthesis. The availability of g-amounts of the synthetic material allowed us to perform stabilization tests on three model enzymes (malate dehydrogenase, staphylococcal nuclease, and lysozyme), and compare the efficacy of MGlyG with other natural glyceryl glycosides, such as α-d-mannosyl-d-glycerate, α-d-glucosyl-d-glycerate and α-d-glucosyl-(1 → 6)-α-d-glucosyl-(1 → 2)-d-glycerate.

Original languageEnglish
Pages (from-to)373-382
Number of pages10
JournalExtremophiles
Volume19
Issue number2
DOIs
Publication statusPublished - 2015

Keywords

  • Compatible solute
  • Glyceryl glycosides
  • Protein stabilization
  • Rubrobacter xylanophilus

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