A solid-phase enzyme linked immunosorbent assay using monoclonal antibodies, for the detection of african swine fever virus antigens and antibodies

Mónica I. Vidal; Matthias Stiene; Jörg Henkel; Ursula Bilitewski; João V. Costa; Abel G. Oliva

doi:10.1016/S0166-0934(97)00059-1

A solid-phase enzyme linked immunosorbent assay using monoclonal antibodies, for the detection of african swine fever virus antigens and antibodies

Mónica I. Vidal, Matthias Stiene, Jörg Henkel, Ursula Bilitewski, João V. Costa, Abel G. Oliva

Instituto de Tecnologia Química e Biológica António Xavier (ITQB)

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

An improved solid-phase enzyme linked immunosorbent assay (ELISA) using monoclonal antibodies was developed to detect an African swine fever virus protein (VP73) in pig samples. The use of monoclonal antibodies against VP73 allowed a sensitive and specific sandwich ELISA. This assay detected a limiting antigen concentration of 0.05 μg/ml of VP73, lower than the detection limit of 0.6 μg/ml obtained by using polyclonal antibodies by the same ELISA. The whole virus particle was detected with this method to a limit of 2.3 x 10² PFU/ml. At the same time, an indirect ELISA was developed to detect ASFV antibodies. The results also indicate that this method may be a useful technique for epidemiological surveys.

Original language	English
Pages (from-to)	211-218
Number of pages	8
Journal	Journal Of Virological Methods
Volume	66
Issue number	2
DOIs	https://doi.org/10.1016/S0166-0934(97)00059-1
Publication status	Published - 1 Jul 1997

Keywords

African swine fever virus
Diagnosis
ELISA
Monoclonal antibodies

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abstract = "An improved solid-phase enzyme linked immunosorbent assay (ELISA) using monoclonal antibodies was developed to detect an African swine fever virus protein (VP73) in pig samples. The use of monoclonal antibodies against VP73 allowed a sensitive and specific sandwich ELISA. This assay detected a limiting antigen concentration of 0.05 μg/ml of VP73, lower than the detection limit of 0.6 μg/ml obtained by using polyclonal antibodies by the same ELISA. The whole virus particle was detected with this method to a limit of 2.3 x 102 PFU/ml. At the same time, an indirect ELISA was developed to detect ASFV antibodies. The results also indicate that this method may be a useful technique for epidemiological surveys.",

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AU - Bilitewski, Ursula

AU - Costa, João V.

AU - Oliva, Abel G.

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A solid-phase enzyme linked immunosorbent assay using monoclonal antibodies, for the detection of african swine fever virus antigens and antibodies

Abstract

Keywords

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