A solid-phase enzyme linked immunosorbent assay using monoclonal antibodies, for the detection of african swine fever virus antigens and antibodies

Mónica I. Vidal, Matthias Stiene, Jörg Henkel, Ursula Bilitewski, João V. Costa, Abel G. Oliva

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

An improved solid-phase enzyme linked immunosorbent assay (ELISA) using monoclonal antibodies was developed to detect an African swine fever virus protein (VP73) in pig samples. The use of monoclonal antibodies against VP73 allowed a sensitive and specific sandwich ELISA. This assay detected a limiting antigen concentration of 0.05 μg/ml of VP73, lower than the detection limit of 0.6 μg/ml obtained by using polyclonal antibodies by the same ELISA. The whole virus particle was detected with this method to a limit of 2.3 x 102 PFU/ml. At the same time, an indirect ELISA was developed to detect ASFV antibodies. The results also indicate that this method may be a useful technique for epidemiological surveys.

Original languageEnglish
Pages (from-to)211-218
Number of pages8
JournalJournal Of Virological Methods
Volume66
Issue number2
DOIs
Publication statusPublished - 1 Jul 1997

Keywords

  • African swine fever virus
  • Diagnosis
  • ELISA
  • Monoclonal antibodies

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