Abstract
This manuscript describes a unique protocol for the rapid transformation of Medicago truncatula A17 cell suspension cultures mediated by Agrobacterium tumefaciens. Medicago cells were collected on day 7 of the growth curve, which corresponded to the beginning of the exponential phase. They were then co-cultured with Agrobacterium for 3 days before being spread onto a petri dish with appropriate antibiotic selection. The Receptor Binding Domain of the Spike protein of SARS-CoV-2 was used as a model to develop this protocol. The presence of the transgene was assessed using PCR, and the integrity of the product was evaluated by SDS-PAGE and Western-blotting.
Original language | English |
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Pages (from-to) | 669-675 |
Number of pages | 7 |
Journal | Plant Cell, Tissue and Organ Culture |
Volume | 153 |
Issue number | 3 |
DOIs | |
Publication status | Published - Jun 2023 |
Keywords
- Agrobacterium
- Cultured cells
- Medicago
- Model legume
- Plant-based system
- RBD
- SARS-CoV-2
- Stable transformation