TY - JOUR
T1 - A Roadmap to Cardiac Tissue-Engineered Construct Preservation
T2 - Insights from Cells, Tissues, and Organs
AU - Sampaio-Pinto, Vasco
AU - Janssen, Jasmijn
AU - Chirico, Nino
AU - Serra, Margarida
AU - Alves, Paula M.
AU - Doevendans, Pieter A.
AU - Voets, Ilja K.
AU - Sluijter, Joost P.G.
AU - van Laake, Linda W.
AU - van Mil, Alain
N1 - Funding Information:
V.S.-P. and J.J. contributed equally to this work. Illustrations in the Graphical Abstract, Figure 1, Figure 3C, Figure 4B and Figure 5 were created with Biorender.com. The work was funded by The Netherlands Heart Foundation (Dekker Senior Clinical Scientist 2019 to L.W.v.L., Grant No. 2019T056); the alliance between Eindhoven University of Technology, Utrecht University and the University Medical Center Utrecht; the European Union (ERC-2014-StG Contract No. 635928); the Dutch Science Foundation (NWO ECHO Grant No. 712.016.002); the project EVICARE (No. 725229) of the European Research Council to J.P.S.; the Gravitation Program ?Materials Driven Regeneration? by the Netherlands Organization for Scientific Research (RegmedXB #024.003.013); and the Marie Sk?odowska-Curie Actions (Grant agreement RESCUE #801540); the Leducq Fondation grant to the Cure-PLaN network; the EU-funded project BRAV? (H2020, ID:874827); the Funda??o para a Ci?ncia e Tecnologia (FCT)-funded project MetaCardio (PTDC/BTMSAL/32566/2017), and iNOVA4Health, UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by FCT/Minist?rio da Ci?ncia, Tecnologia e Ensino Superior, through Portuguese national funds. Note: The article number in ref. [18] was added on July 6, 2021, after initial publication online.
Funding Information:
V.S.‐P. and J.J. contributed equally to this work. Illustrations in the Graphical Abstract, Figure 1, Figure 3C, Figure 4B and Figure 5 were created with Biorender.com. The work was funded by The Netherlands Heart Foundation (Dekker Senior Clinical Scientist 2019 to L.W.v.L., Grant No. 2019T056); the alliance between Eindhoven University of Technology, Utrecht University and the University Medical Center Utrecht; the European Union (ERC‐2014‐StG Contract No. 635928); the Dutch Science Foundation (NWO ECHO Grant No. 712.016.002); the project EVICARE (No. 725229) of the European Research Council to J.P.S.; the Gravitation Program “Materials Driven Regeneration” by the Netherlands Organization for Scientific Research (RegmedXB #024.003.013); and the Marie Skłodowska‐Curie Actions (Grant agreement RESCUE #801540); the Leducq Fondation grant to the Cure‐PLaN network; the EU‐funded project BRAV∃ (H2020, ID:874827); the Fundação para a Ciência e Tecnologia (FCT)‐funded project MetaCardio (PTDC/BTMSAL/32566/2017), and iNOVA4Health, UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by FCT/Ministério da Ciência, Tecnologia e Ensino Superior, through Portuguese national funds.
Publisher Copyright:
© 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH.
PY - 2021/7/8
Y1 - 2021/7/8
N2 - Worldwide, over 26 million patients suffer from heart failure (HF). One strategy aspiring to prevent or even to reverse HF is based on the transplantation of cardiac tissue-engineered (cTE) constructs. These patient-specific constructs aim to closely resemble the native myocardium and, upon implantation on the diseased tissue, support and restore cardiac function, thereby preventing the development of HF. However, cTE constructs off-the-shelf availability in the clinical arena critically depends on the development of efficient preservation methodologies. Short- and long-term preservation of cTE constructs would enable transportation and direct availability. Herein, currently available methods, from normothermic- to hypothermic- to cryopreservation, for the preservation of cardiomyocytes, whole-heart, and regenerative materials are reviewed. A theoretical foundation and recommendations for future research on developing cTE construct specific preservation methods are provided. Current research suggests that vitrification can be a promising procedure to ensure long-term cryopreservation of cTE constructs, despite the need of high doses of cytotoxic cryoprotective agents. Instead, short-term cTE construct preservation can be achieved at normothermic or hypothermic temperatures by administration of protective additives. With further tuning of these promising methods, it is anticipated that cTE construct therapy can be brought one step closer to the patient.
AB - Worldwide, over 26 million patients suffer from heart failure (HF). One strategy aspiring to prevent or even to reverse HF is based on the transplantation of cardiac tissue-engineered (cTE) constructs. These patient-specific constructs aim to closely resemble the native myocardium and, upon implantation on the diseased tissue, support and restore cardiac function, thereby preventing the development of HF. However, cTE constructs off-the-shelf availability in the clinical arena critically depends on the development of efficient preservation methodologies. Short- and long-term preservation of cTE constructs would enable transportation and direct availability. Herein, currently available methods, from normothermic- to hypothermic- to cryopreservation, for the preservation of cardiomyocytes, whole-heart, and regenerative materials are reviewed. A theoretical foundation and recommendations for future research on developing cTE construct specific preservation methods are provided. Current research suggests that vitrification can be a promising procedure to ensure long-term cryopreservation of cTE constructs, despite the need of high doses of cytotoxic cryoprotective agents. Instead, short-term cTE construct preservation can be achieved at normothermic or hypothermic temperatures by administration of protective additives. With further tuning of these promising methods, it is anticipated that cTE construct therapy can be brought one step closer to the patient.
KW - antifreeze proteins
KW - cardiac tissue engineering
KW - cryopreservation
KW - cryoprotective agents
KW - heart failure
KW - hypothermic and normothermic preservation
KW - vitrification
UR - http://www.scopus.com/inward/record.url?scp=85106692936&partnerID=8YFLogxK
U2 - 10.1002/adma.202008517
DO - 10.1002/adma.202008517
M3 - Review article
C2 - 34048090
AN - SCOPUS:85106692936
SN - 0935-9648
VL - 33
JO - Advanced Materials
JF - Advanced Materials
IS - 27
M1 - 2008517
ER -