The current research trend is finding out feasible solutions to guarantee sustainable building practices. Among the studied possibilities, the production of eco-friendly materials is widely investigated. To propose efficient building products the proper evaluation of their properties is extremely important. The present work considers some laboratory tests to analyse building materials, focusing on agro-industrial wastes (bio-wastes of floriculture and forest management, agricultural practices and agro-industrial processes). It considers hygrothermal performance and biological susceptibility of agro-industrial wastes both individually and in composites. Several past studies addressed this topic by using different laboratory tests and mainly considering composites. Although bio-susceptibility is one of the main drawbacks of bio-based building materials, it is not so commonly assessed. However, many laboratory tests can be carried out, due to the different bio agents and critical conditions. The present work aims at collecting this information to give a summarised list of methods that could be a simplified starting point to study agro-industrial wastes and agro-industrial waste-based composites for building practices.