A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide

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Abstract

The ubiquitous nitrite is a major analyte in the management of human health and environmental risks. The current analytical methods are complex techniques that do not fulfil the need for simple, robust and low-cost tools for on-site monitoring. Electrochemical reductase-based biosensors are presented as a powerful alternative, due to their good analytical performance and miniaturization potential. However, their real-world application is limited by the need of anoxic working conditions, and the standard oxygen removal strategies are incompatible with point-of-care measurements. Instead, a bienzymatic oxygen scavenger system comprising glucose oxidase and catalase can be used to promote anoxic conditions in aired environments. Herein, carbon screen-printed electrodes were modified with cytochrome c nitrite reductase together with glucose oxidase and catalase, so that nitrite cathodic detection could be performed by cyclic voltammetry under ambient air. The resulting biosensor displayed good linear response to the analyte (2–200 µM, sensitivity of 326 ± 5 mA M −1 cm −2 at −0.8 V; 0.8–150 µM, sensitivity of 511 ± 11 mA M −1 cm −2 at −0.5 V), while being free from oxygen interference and stable up to 1 month. Furthermore, the biosensor’s catalytic response was unaffected by the presence of cyanide, a well-known inhibitor of heme-enzymes.

Original languageEnglish
Article number2622
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

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Point-of-Care Systems
Cyanides
Biosensing Techniques
Nitrites
Glucose Oxidase
nitrate reductase (cytochrome)
Oxygen
Catalase
Miniaturization
Environmental Health
Enzyme Inhibitors
Heme
Oxidoreductases
Electrodes
Carbon
Air
Costs and Cost Analysis

Cite this

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title = "A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide",
abstract = "The ubiquitous nitrite is a major analyte in the management of human health and environmental risks. The current analytical methods are complex techniques that do not fulfil the need for simple, robust and low-cost tools for on-site monitoring. Electrochemical reductase-based biosensors are presented as a powerful alternative, due to their good analytical performance and miniaturization potential. However, their real-world application is limited by the need of anoxic working conditions, and the standard oxygen removal strategies are incompatible with point-of-care measurements. Instead, a bienzymatic oxygen scavenger system comprising glucose oxidase and catalase can be used to promote anoxic conditions in aired environments. Herein, carbon screen-printed electrodes were modified with cytochrome c nitrite reductase together with glucose oxidase and catalase, so that nitrite cathodic detection could be performed by cyclic voltammetry under ambient air. The resulting biosensor displayed good linear response to the analyte (2–200 µM, sensitivity of 326 ± 5 mA M −1 cm −2 at −0.8 V; 0.8–150 µM, sensitivity of 511 ± 11 mA M −1 cm −2 at −0.5 V), while being free from oxygen interference and stable up to 1 month. Furthermore, the biosensor’s catalytic response was unaffected by the presence of cyanide, a well-known inhibitor of heme-enzymes.",
author = "Tiago Monteiro and Sara Gomes and Elena Jubete and Larraitz A{\~n}orga and Silveira, {C{\'e}lia M.} and Almeida, {Maria Gabriela}",
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A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide. / Monteiro, Tiago; Gomes, Sara; Jubete, Elena; Añorga, Larraitz; Silveira, Célia M.; Almeida, Maria Gabriela.

In: Scientific Reports, Vol. 9, No. 1, 2622, 01.12.2019.

Research output: Contribution to journalArticle

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