A process engineering approach to improve production of P(3HB) by cupriavidus necator from used cooking oil

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Abstract

Different feeding strategies, namely, exponential feeding and DO-stat mode, were implemented for the production of poly(3-hydroxybutyrate), P(3HB), by Cupriavidus necator DSM 428 with used cooking oil (UCO) as the sole carbon source. With the exponential feeding strategy, a cell dry mass of 21.3 ± 0.9 g L −1 was obtained, with a polymer content of 84.0 ± 4.5 wt.%, giving an overall volumetric productivity of 4.5 ± 0.2 g L −1 day −1 . However, the highest P(3HB) volumetric productivity, 12.6 ± 0.8 g L −1 day −1 , was obtained when the DO-stat mode was implemented together with the use of ammonium hydroxide for pH control, which served as an additional nitrogen source and allowed to reach higher cell dry mass (7.8 ± 0.6 g L −1 ). The P(3HB) obtained in all experiments had a high molecular mass, ranging from 0.6 × 10 5 to 2.6 × 10 5 g mol −1 , with low polydispersity indexes of 1.2-1.6. Melting and glass transition temperatures were also similar for the polymer produced with both cultivation strategy, 174 ° C and 3.0-4.0 ° C, respectively. The polymer exhibited a crystallinity ranging from 52 to 65%. The DO-stat strategy to feed oil containing substrates as the sole carbon sources was reported for the first time in this study, and the preliminary results obtained show that it is a promising strategy to improve P(3HB) production. Nevertheless, the process requires further optimization in order to make it economically viable.

Original languageEnglish
Article number2191650
JournalInternational Journal of Polymer Science
Volume2019
DOIs
Publication statusPublished - 1 Jan 2019

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3-Hydroxybutyric Acid
Cooking
Process engineering
Polymers
Oils
Carbon
Productivity
Ammonium Hydroxide
Ammonium hydroxide
Molecular mass
Polydispersity
Melting
Nitrogen
Substrates
Experiments

Cite this

@article{178492fd97b44c2895b4bb153ef0279f,
title = "A process engineering approach to improve production of P(3HB) by cupriavidus necator from used cooking oil",
abstract = "Different feeding strategies, namely, exponential feeding and DO-stat mode, were implemented for the production of poly(3-hydroxybutyrate), P(3HB), by Cupriavidus necator DSM 428 with used cooking oil (UCO) as the sole carbon source. With the exponential feeding strategy, a cell dry mass of 21.3 ± 0.9 g L −1 was obtained, with a polymer content of 84.0 ± 4.5 wt.{\%}, giving an overall volumetric productivity of 4.5 ± 0.2 g L −1 day −1 . However, the highest P(3HB) volumetric productivity, 12.6 ± 0.8 g L −1 day −1 , was obtained when the DO-stat mode was implemented together with the use of ammonium hydroxide for pH control, which served as an additional nitrogen source and allowed to reach higher cell dry mass (7.8 ± 0.6 g L −1 ). The P(3HB) obtained in all experiments had a high molecular mass, ranging from 0.6 × 10 5 to 2.6 × 10 5 g mol −1 , with low polydispersity indexes of 1.2-1.6. Melting and glass transition temperatures were also similar for the polymer produced with both cultivation strategy, 174 ° C and 3.0-4.0 ° C, respectively. The polymer exhibited a crystallinity ranging from 52 to 65{\%}. The DO-stat strategy to feed oil containing substrates as the sole carbon sources was reported for the first time in this study, and the preliminary results obtained show that it is a promising strategy to improve P(3HB) production. Nevertheless, the process requires further optimization in order to make it economically viable.",
author = "Cruz, {Madalena V.} and Gouveia, {Ana Rosa} and Madalena Dion{\'i}sio and Filomena Freitas and Reis, {Maria A. M.}",
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T1 - A process engineering approach to improve production of P(3HB) by cupriavidus necator from used cooking oil

AU - Cruz, Madalena V.

AU - Gouveia, Ana Rosa

AU - Dionísio, Madalena

AU - Freitas, Filomena

AU - Reis, Maria A. M.

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N2 - Different feeding strategies, namely, exponential feeding and DO-stat mode, were implemented for the production of poly(3-hydroxybutyrate), P(3HB), by Cupriavidus necator DSM 428 with used cooking oil (UCO) as the sole carbon source. With the exponential feeding strategy, a cell dry mass of 21.3 ± 0.9 g L −1 was obtained, with a polymer content of 84.0 ± 4.5 wt.%, giving an overall volumetric productivity of 4.5 ± 0.2 g L −1 day −1 . However, the highest P(3HB) volumetric productivity, 12.6 ± 0.8 g L −1 day −1 , was obtained when the DO-stat mode was implemented together with the use of ammonium hydroxide for pH control, which served as an additional nitrogen source and allowed to reach higher cell dry mass (7.8 ± 0.6 g L −1 ). The P(3HB) obtained in all experiments had a high molecular mass, ranging from 0.6 × 10 5 to 2.6 × 10 5 g mol −1 , with low polydispersity indexes of 1.2-1.6. Melting and glass transition temperatures were also similar for the polymer produced with both cultivation strategy, 174 ° C and 3.0-4.0 ° C, respectively. The polymer exhibited a crystallinity ranging from 52 to 65%. The DO-stat strategy to feed oil containing substrates as the sole carbon sources was reported for the first time in this study, and the preliminary results obtained show that it is a promising strategy to improve P(3HB) production. Nevertheless, the process requires further optimization in order to make it economically viable.

AB - Different feeding strategies, namely, exponential feeding and DO-stat mode, were implemented for the production of poly(3-hydroxybutyrate), P(3HB), by Cupriavidus necator DSM 428 with used cooking oil (UCO) as the sole carbon source. With the exponential feeding strategy, a cell dry mass of 21.3 ± 0.9 g L −1 was obtained, with a polymer content of 84.0 ± 4.5 wt.%, giving an overall volumetric productivity of 4.5 ± 0.2 g L −1 day −1 . However, the highest P(3HB) volumetric productivity, 12.6 ± 0.8 g L −1 day −1 , was obtained when the DO-stat mode was implemented together with the use of ammonium hydroxide for pH control, which served as an additional nitrogen source and allowed to reach higher cell dry mass (7.8 ± 0.6 g L −1 ). The P(3HB) obtained in all experiments had a high molecular mass, ranging from 0.6 × 10 5 to 2.6 × 10 5 g mol −1 , with low polydispersity indexes of 1.2-1.6. Melting and glass transition temperatures were also similar for the polymer produced with both cultivation strategy, 174 ° C and 3.0-4.0 ° C, respectively. The polymer exhibited a crystallinity ranging from 52 to 65%. The DO-stat strategy to feed oil containing substrates as the sole carbon sources was reported for the first time in this study, and the preliminary results obtained show that it is a promising strategy to improve P(3HB) production. Nevertheless, the process requires further optimization in order to make it economically viable.

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U2 - 10.1155/2019/2191650

DO - 10.1155/2019/2191650

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VL - 2019

JO - International Journal of Polymer Science

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