A Novel Nitroreductase of Staphylococcus aureus with S-Nitrosoglutathione Reductase Activity

Ligia Nobre; Ligia Raquel Teixeira

doi:10.1128/jb.00022-09

A Novel Nitroreductase of Staphylococcus aureus with S-Nitrosoglutathione Reductase Activity

Ligia Nobre, Ligia Raquel Teixeira

Instituto de Tecnologia Química e Biológica António Xavier (ITQB)

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

In this report we show that inactivation of the putative nitroreductase SA0UHSC_00833 (ntrA) increases the sensitivity of Staphylococcus aureus to S-nitrosoglutathione (GSNO) and augments its resistance to nitrofurans. S. aureus NtrA is a bifunctional enzyme that exhibits nitroreductase and GSNO reductase activity. A phylogenetic analysis suggests that NtrA is a member of a novel family of nitroreductases that seems to play a dual role in vivo, promoting nitrofuran activation and protecting the cell against transnitrosylation.

Original language	Unknown
Pages (from-to)	3403-3406
Journal	Journal of Bacteriology
Volume	191
Issue number	10
DOIs	https://doi.org/10.1128/jb.00022-09
Publication status	Published - 1 Jan 2009

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10.1128/jb.00022-09

Cite this

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title = "A Novel Nitroreductase of Staphylococcus aureus with S-Nitrosoglutathione Reductase Activity",

abstract = "In this report we show that inactivation of the putative nitroreductase SA0UHSC\_00833 (ntrA) increases the sensitivity of Staphylococcus aureus to S-nitrosoglutathione (GSNO) and augments its resistance to nitrofurans. S. aureus NtrA is a bifunctional enzyme that exhibits nitroreductase and GSNO reductase activity. A phylogenetic analysis suggests that NtrA is a member of a novel family of nitroreductases that seems to play a dual role in vivo, promoting nitrofuran activation and protecting the cell against transnitrosylation.",

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doi = "10.1128/jb.00022-09",

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AU - Nobre, Ligia

AU - Teixeira, Ligia Raquel

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N2 - In this report we show that inactivation of the putative nitroreductase SA0UHSC_00833 (ntrA) increases the sensitivity of Staphylococcus aureus to S-nitrosoglutathione (GSNO) and augments its resistance to nitrofurans. S. aureus NtrA is a bifunctional enzyme that exhibits nitroreductase and GSNO reductase activity. A phylogenetic analysis suggests that NtrA is a member of a novel family of nitroreductases that seems to play a dual role in vivo, promoting nitrofuran activation and protecting the cell against transnitrosylation.

AB - In this report we show that inactivation of the putative nitroreductase SA0UHSC_00833 (ntrA) increases the sensitivity of Staphylococcus aureus to S-nitrosoglutathione (GSNO) and augments its resistance to nitrofurans. S. aureus NtrA is a bifunctional enzyme that exhibits nitroreductase and GSNO reductase activity. A phylogenetic analysis suggests that NtrA is a member of a novel family of nitroreductases that seems to play a dual role in vivo, promoting nitrofuran activation and protecting the cell against transnitrosylation.

KW - glutathione

KW - decomposition

KW - enzyme

KW - system

KW - stress-response

KW - infections

KW - resistance

KW - nitrosothiols

U2 - 10.1128/jb.00022-09

DO - 10.1128/jb.00022-09

M3 - Article

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VL - 191

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EP - 3406

JO - Journal of Bacteriology

JF - Journal of Bacteriology

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ER -