A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas

Rute Rodrigues, Filipa M.A. Valente, Inês A.C. Pereira, Solange Oliveira, Claudina Rodrigues-Pousada

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

In the present study, we report the identification of an operon with six coding regions for a multisubunit membrane-bound [NiFe] hydrogenase in the genome of Desulfovibrio gigas. Sequence analysis of the deduced polypeptides reveals a high similarity to subunits of proteins belonging to the family of Ech hydrogenases. The operon is organised similarly to the operon coding for the Ech hydrogenase from Methanosarcina barkeri, suggesting that both encode very similar hydrogenases. Expression of the operon was detected by Northern blot and RT-PCR analyses, and the presence of the encoded proteins was examined by Western blotting. The possible role of this hydrogenase is discussed, relating it with a potential function in the H2 cycling as a mechanism for energy conservation in D. gigas. The present study provides therefore valuable insights into the open question of the energy conserving mechanism in D. gigas.

Original languageEnglish
Pages (from-to)366-375
Number of pages10
JournalBiochemical And Biophysical Research Communications
Volume306
Issue number2
DOIs
Publication statusPublished - 27 Jun 2003

Keywords

  • Desulfovibrio gigas
  • Ech hydrogenase
  • Energy conservation
  • H cycling

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