A newfangled collagenase inhibitor topical formulation based on ethosomes with sambucus nigra l. Extract

Ana Henriques Mota, Inês Prazeres, Henrique Mestre, Andreia Bento-Silva, Maria João Rodrigues, Noélia Duarte, Ana Teresa Serra, Maria Rosário Bronze, Patrícia Rijo, Maria Manuela Gaspar, Ana Silveira Viana, Lia Ascensão, Pedro Pinto, Pradeep Kumar, António José Almeida, Catarina Pinto Reis

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Sambucus nigra L. (S. nigra) is a shrub widespread in Europe and western Asia, traditionally used in medicine, that has become popular in recent years as a potential source of a wide range of interesting bioactive compounds. The aim of the present work was to develop a topical S. nigra extract formulation based on ethosomes and thus to support its health claims with scientific evidence. S. nigra extract was prepared by an ultrasound-assisted method and then included in ethosomes. The ethosomes were analyzed in terms of their size, stability over time, morphology, entrapment capacity (EC), extract release profile, stability over time and several biological activities. The prepared ethosomes were indicated to be well defined, presenting sizes around 600 nm. The extract entrapment capacity in ethosomes was 73.9 ± 24.8%, with an interesting slow extract release profile over 24 h. The extract-loaded ethosomes presented collagenase inhibition activity and a very good skin compatibility after human application. This study demonstrates the potential use of S. nigra extract incorporated in ethosomes as a potential cosmeceutical ingredient and on further studies should be performed to better understand the impact of S. nigra compounds on skin care over the time.

Original languageEnglish
Article number467
Issue number5
Publication statusPublished - May 2021


  • Collagenase inhibition
  • Ethosomes
  • Sambucus nigra L
  • Skin compatibility


Dive into the research topics of 'A newfangled collagenase inhibitor topical formulation based on ethosomes with sambucus nigra l. Extract'. Together they form a unique fingerprint.

Cite this