Abstract
The objective of this study was to develop a Rapid Insulin Sensitivity Test (RIST) in humans, a test already used in animal studies. Insulin sensitivity was assessed using a rapid modified euglycemic clamp, the RIST. In this test, glucose disposition was determined after an intravenous (IV) bolus (50mU/kg bw administered over 30 seconds) of insulin, before and after feeding a standardized test meal, in healthy male subjects (aged 27.8±2.4 years, BMI 23.5±1.2 kg/m2). The RIST uses as the index of insulin sensitivity, the total amount of glucose required to be infused to maintain euglycemia during insulin action following an IV bolus of insulin. During the RIST, glucose levels are determined at 2-min intervals in order to clamp the glycemia at baseline values. Following a 24 hr fasting period, the RIST index was 225.6±25.1 mg glucose/kg bw. The volunteers were then fed a standardized test meal, a new stable glucose level was obtained 100 min after the meal, and a second RIST was performed. The glucose requirement (RIST index) increased to 647.9±73.5 mg glucose/kg bw following the standardized test meal (n=5, p<0.001). This report describes a new technique to evaluate insulin sensitivity in healthy humans. The RIST is a powerful research tool to assess the glucose utilization action of an insulin bolus in fasted and fed states both evaluated in the same day.
| Original language | English |
|---|---|
| Pages (from-to) | 105-109 |
| Number of pages | 5 |
| Journal | Proceedings Of The Western Pharmacology Society |
| Volume | 50 |
| Publication status | Published - 1 Dec 2007 |
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A new technique to assess insulin sensitivity in humans : The Rapid Insulin Sensitivity Test (RIST). / Patarrão, Rita S.; Wayne Lautt, W.; Guarino, Maria P.; Afonso, Ricardo A.; Ribeiro, Rogério T.; Fernandes, Ana B.; Boavida, José M.; Macedo, Maria Paula.
In: Proceedings Of The Western Pharmacology Society, Vol. 50, 01.12.2007, p. 105-109.Research output: Contribution to journal › Article
TY - JOUR
T1 - A new technique to assess insulin sensitivity in humans
T2 - The Rapid Insulin Sensitivity Test (RIST)
AU - Patarrão, Rita S.
AU - Wayne Lautt, W.
AU - Guarino, Maria P.
AU - Afonso, Ricardo A.
AU - Ribeiro, Rogério T.
AU - Fernandes, Ana B.
AU - Boavida, José M.
AU - Macedo, Maria Paula
PY - 2007/12/1
Y1 - 2007/12/1
N2 - The objective of this study was to develop a Rapid Insulin Sensitivity Test (RIST) in humans, a test already used in animal studies. Insulin sensitivity was assessed using a rapid modified euglycemic clamp, the RIST. In this test, glucose disposition was determined after an intravenous (IV) bolus (50mU/kg bw administered over 30 seconds) of insulin, before and after feeding a standardized test meal, in healthy male subjects (aged 27.8±2.4 years, BMI 23.5±1.2 kg/m2). The RIST uses as the index of insulin sensitivity, the total amount of glucose required to be infused to maintain euglycemia during insulin action following an IV bolus of insulin. During the RIST, glucose levels are determined at 2-min intervals in order to clamp the glycemia at baseline values. Following a 24 hr fasting period, the RIST index was 225.6±25.1 mg glucose/kg bw. The volunteers were then fed a standardized test meal, a new stable glucose level was obtained 100 min after the meal, and a second RIST was performed. The glucose requirement (RIST index) increased to 647.9±73.5 mg glucose/kg bw following the standardized test meal (n=5, p<0.001). This report describes a new technique to evaluate insulin sensitivity in healthy humans. The RIST is a powerful research tool to assess the glucose utilization action of an insulin bolus in fasted and fed states both evaluated in the same day.
AB - The objective of this study was to develop a Rapid Insulin Sensitivity Test (RIST) in humans, a test already used in animal studies. Insulin sensitivity was assessed using a rapid modified euglycemic clamp, the RIST. In this test, glucose disposition was determined after an intravenous (IV) bolus (50mU/kg bw administered over 30 seconds) of insulin, before and after feeding a standardized test meal, in healthy male subjects (aged 27.8±2.4 years, BMI 23.5±1.2 kg/m2). The RIST uses as the index of insulin sensitivity, the total amount of glucose required to be infused to maintain euglycemia during insulin action following an IV bolus of insulin. During the RIST, glucose levels are determined at 2-min intervals in order to clamp the glycemia at baseline values. Following a 24 hr fasting period, the RIST index was 225.6±25.1 mg glucose/kg bw. The volunteers were then fed a standardized test meal, a new stable glucose level was obtained 100 min after the meal, and a second RIST was performed. The glucose requirement (RIST index) increased to 647.9±73.5 mg glucose/kg bw following the standardized test meal (n=5, p<0.001). This report describes a new technique to evaluate insulin sensitivity in healthy humans. The RIST is a powerful research tool to assess the glucose utilization action of an insulin bolus in fasted and fed states both evaluated in the same day.
UR - http://www.scopus.com/inward/record.url?scp=47749092863&partnerID=8YFLogxK
M3 - Article
VL - 50
SP - 105
EP - 109
JO - Proceedings Of The Western Pharmacology Society
JF - Proceedings Of The Western Pharmacology Society
SN - 0083-8969
ER -