A New Method for the Chiral HRGC Assay of L-2-Hydroxyglutaric Acid in Urine

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Abstract

L-2-Hydroxyglutaric aciduria is a recently discovered inherited neurometabolic disease for which diagnostic identification of the right enantiomer is required. A method is described where alkyl esters of both L- and D-2-hydroxyglutaric acids are prepared without side lactonization. Esterification is achieved at room temperature by reaction with appropriate alkyl chloroformates. This method avoids lactonization, in contrast to esterification with alcohols in acidic media or with diazomethane. The identity of the derivatives is established by HRGC-FTIR and HRGC-MS. Chiral HRGC separation with capillaries coated with 1(R)-trans-N-N′-1,2-cyclohexylenebisbenzamideoligodimethyL-siloxane and with a cyclodextrin is compared. The method is adequate for quantitative determinations.

Original languageEnglish
Pages (from-to)161-164
Number of pages4
JournalHRC Journal of High Resolution Chromatography
Volume19
Issue number3
Publication statusPublished - 1 Mar 1996

Keywords

  • 1(R)-trans-N,N′-1,2-cyclohexenylbisbenzamideoligosiloxane
  • Chiral HRGC
  • Chiral liquid phases
  • Cyclodextrins
  • L-2-Hydroxyglutaric acid
  • L-2-Hydroxyglutaric aciduria

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