A New Genotype 2 Subcluster Identified Among GBV-C Strains Circulating in the Lisbon Metropolitan Area of Portugal

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Abstract

The rate of infection by the GBV-C virus was investigated in a group of 214 individuals at high risk of infection with parenterally transmitted viruses, and all living in the Lisbon metropolitan area (Portugal). RNA was extracted from plasma samples, and a fragment of the 5'-UTR was amplified by RT-PCR, disclosing a high prevalence of infection (40.7%). Most probably due to similar modes of viral transmission, the majority of GBV-C (+) individuals were found to be coinfected with HIV and/or HCV. A genomic region covering part of the E1/E2 glycoprotein coding sequence was amplified from approximately half of the GBV-C positive samples (44/87). Phylogenetic analysis of nucleotide sequences showed segregation of Portuguese GBV-C strains with genotype 1 (G1, n = 10) and genotype 2 (G2, n = 24) references. Genotype 1 was significantly associated with the African descent of those infected. Curiously, some of the strains assigned to genotype 2 were shown to form a separate cluster (designated G2*) in both neighbor-joining and Bayesian phylogenetic trees, which was confirmed by multivariate principal coordinate analysis. However, analysis of the distribution of intra- and intergenotype genetic distances support the hypothesis that rather than corresponding to a new viral genotype, G2* is a geographical subduster within the genotype 2 radiation. J. Med. Virol. 82:452-459,2010. (C) 2010 Wiley-Liss, Inc.
Original languageEnglish
Pages (from-to)452-459
JournalJournal Of Medical Virology
Volume82
Issue number3
DOIs
Publication statusPublished - 1 Jan 2010

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  • SDG 3 - Good Health and Well-Being

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