A New Biosurfactant/Bioemulsifier from Gordonia alkanivorans Strain 1B: Production and Characterization

Tiago P. Silva, Susana M. Paixão, João Tavares, Cátia V. Gil, Cristiana A. V. Torres, Filomena Freitas, Luís Alves

Research output: Contribution to journalArticlepeer-review

Abstract

Biosurfactants and bioemulsifiers (BS/BE) are naturally synthesized molecules, which can be used as alternatives to traditional detergents. These molecules are commonly produced by microorganisms isolated from hydrocarbon-rich environments. Gordonia alkanivorans strain 1B was originally found in such an environment, however little was known about its abilities as a BS/BE producer. The goal of this work was to access the potential of strain 1B as a BS/BE producer and perform the initial characterization of the produced compounds. It was demonstrated that strain 1B was able to synthesize lipoglycoprotein compounds with BS/BE properties, both extracellularly and adhered to the cells, without the need for a hydrophobic inducer, producing emulsion in several different hydrophobic phases. Using a crude BS/BE powder, the critical micelle concentration was determined (CMC = 16.94 mg/L), and its capacity to reduce the surface tension to a minimum of 35.63 mN/m was demonstrated, surpassing many commercial surfactants. Moreover, after dialysis, emulsification assays revealed an activity similar to that of Triton X-100 in almond and sunflower oils. In benzene, the E24 value attained was 83.45%, which is 30% greater than that of the commercial alternative. The results obtained highlight for the presence of promising novel BS/BE produced by strain 1B.

Original languageEnglish
Article number845
Number of pages18
JournalProcesses
Volume10
Issue number5
DOIs
Publication statusPublished - 25 Apr 2022

Keywords

  • critical micelle concentration
  • emulsification index
  • G. alkanivorans strain 1B
  • new biosurfactant/bioemulsifier
  • surface tension

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