A Multiwell-Based Detection Platform with Integrated PDMS Concentrators for Rapid Multiplexed Enzymatic Assays

Xi Wei, Vu Q Do, Sang V Pham, Diogo Martins, Yong-Ak Song

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
25 Downloads (Pure)

Abstract

We report an integrated system for accelerating assays with concentrators in a standard 12-well plate (ISAAC-12) and demonstrate its versatility for rapid detection of matrix metalloproteinase (MMP)-9 expression in the cell culture supernatant of breast cancer cell line MDA-MB-231 by accelerating the enzymatic reaction and end-point signal intensity via electrokinetic preconcentration. Using direct printing of a conductive ion-permselective polymer on a polydimethylsiloxane (PDMS) channel, the new microfluidic concentrator chip can be built without modifying the underlying substrate. Through this decoupling fabrication strategy, our microfluidic concentrator chip can easily be integrated with a standard multiwell plate, the de facto laboratory standard platform for high-throughput assays, simply by reversible bonding on the bottom of each well. It increases the reaction rate of enzymatic assays by concentrating the enzyme and the reaction product inside each well simultaneously for rapid multiplexed detection.

Original languageEnglish
Article number10772
Number of pages11
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 17 Jul 2018

Keywords

  • Assay systems
  • Diagnostic markers
  • Surface patterning

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