A multiproxy approach to evaluate biocidal treatments on biodeteriorated majolica glazed tiles

Mathilda L. Coutinho, A. Z. Miller, P. M. Martin-Sanchez, J. Mirão, A. Gomez-Bolea, B. Machado-Moreira, L. Cerqueira-Alves, V. Jurado, C. Saiz-Jimenez, A. Lima, A. J L Phillips, F. Pina, Maria Filomena Meireles Abrantes de Macedo Dinis

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The Fishing House located on the grounds of the Marquis of Pombal Palace, Oeiras, Portugal, was built in the 18th century. During this epoch, Portuguese gardens, such as the one surrounding the Fishing House, were commonly ornamented with glazed wall tile claddings. Currently, some of these outdoor tile panels are covered with dark colored biofilms, contributing to undesirable aesthetic changes and eventually inducing chemical and physical damage to the tile surfaces. Phylogenetic analyses revealed that the investigated biofilms are mainly composed of green algae, cyanobacteria and dematiaceous fungi. With the aim of mitigating biodeterioration, four different biocides (TiO2 nanoparticles, Biotin® T, Preventol® RI 80 and Albilex Biostat®) were applied in situ to the glazed wall tiles. Their efficacy was monitored by visual examination, epifluorescence microscopy and DNA-based analysis. Significant changes in the microbial community composition were observed 4 months after treatment with Preventol® RI 80 and Biotin® T. Although the original community was inactivated after these treatments, an early stage of re-colonization was detected 6 months after the biocide application. TiO2 nanoparticles showed promising results due to their self-cleaning effect, causing the detachment of the biofilm from the tile surface, which remained clean 6 and even 24 months after biocide application.

Original languageEnglish
JournalEnvironmental Microbiology
DOIs
Publication statusPublished - Dec 2016

Keywords

  • GRADIENT GEL-ELECTROPHORESIS
  • 16S RIBOSOMAL-RNA
  • BLACK FUNGI
  • PHYCOPELTIS TRENTEPOHLIACEAE
  • MOLECULAR TECHNIQUES
  • ANTIQUE MARBLES
  • CARBONATE ROCK
  • DIVERSITY

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