TY - JOUR
T1 - A multiproxy approach to evaluate biocidal treatments on biodeteriorated majolica glazed tiles
AU - Coutinho, Mathilda L.
AU - Miller, A. Z.
AU - Martin-Sanchez, P. M.
AU - Mirão, J.
AU - Gomez-Bolea, A.
AU - Machado-Moreira, B.
AU - Cerqueira-Alves, L.
AU - Jurado, V.
AU - Saiz-Jimenez, C.
AU - Lima, A.
AU - Phillips, A. J L
AU - Pina, F.
AU - Dinis, Maria Filomena Meireles Abrantes de Macedo
N1 - Sem PDF conforme despacho.
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F46038%2F2008/PT#
info:eu-repo/grantAgreement/FCT/5876/147238/PT#
Portuguese Foundation for Science and Technology (FCT-MCTES) -(SFRH/BD/46038/2008; UID/EAT/00729/2013 );
European Commission (PIEF-GA-2012-328689);
Spanish Ministry of Science and Innovation (TCP CSD2007-00058).
PY - 2016/12
Y1 - 2016/12
N2 - The Fishing House located on the grounds of the Marquis of Pombal Palace, Oeiras, Portugal, was built in the 18th century. During this epoch, Portuguese gardens, such as the one surrounding the Fishing House, were commonly ornamented with glazed wall tile claddings. Currently, some of these outdoor tile panels are covered with dark colored biofilms, contributing to undesirable aesthetic changes and eventually inducing chemical and physical damage to the tile surfaces. Phylogenetic analyses revealed that the investigated biofilms are mainly composed of green algae, cyanobacteria and dematiaceous fungi. With the aim of mitigating biodeterioration, four different biocides (TiO2 nanoparticles, Biotin® T, Preventol® RI 80 and Albilex Biostat®) were applied in situ to the glazed wall tiles. Their efficacy was monitored by visual examination, epifluorescence microscopy and DNA-based analysis. Significant changes in the microbial community composition were observed 4 months after treatment with Preventol® RI 80 and Biotin® T. Although the original community was inactivated after these treatments, an early stage of re-colonization was detected 6 months after the biocide application. TiO2 nanoparticles showed promising results due to their self-cleaning effect, causing the detachment of the biofilm from the tile surface, which remained clean 6 and even 24 months after biocide application.
AB - The Fishing House located on the grounds of the Marquis of Pombal Palace, Oeiras, Portugal, was built in the 18th century. During this epoch, Portuguese gardens, such as the one surrounding the Fishing House, were commonly ornamented with glazed wall tile claddings. Currently, some of these outdoor tile panels are covered with dark colored biofilms, contributing to undesirable aesthetic changes and eventually inducing chemical and physical damage to the tile surfaces. Phylogenetic analyses revealed that the investigated biofilms are mainly composed of green algae, cyanobacteria and dematiaceous fungi. With the aim of mitigating biodeterioration, four different biocides (TiO2 nanoparticles, Biotin® T, Preventol® RI 80 and Albilex Biostat®) were applied in situ to the glazed wall tiles. Their efficacy was monitored by visual examination, epifluorescence microscopy and DNA-based analysis. Significant changes in the microbial community composition were observed 4 months after treatment with Preventol® RI 80 and Biotin® T. Although the original community was inactivated after these treatments, an early stage of re-colonization was detected 6 months after the biocide application. TiO2 nanoparticles showed promising results due to their self-cleaning effect, causing the detachment of the biofilm from the tile surface, which remained clean 6 and even 24 months after biocide application.
KW - GRADIENT GEL-ELECTROPHORESIS
KW - 16S RIBOSOMAL-RNA
KW - BLACK FUNGI
KW - PHYCOPELTIS TRENTEPOHLIACEAE
KW - MOLECULAR TECHNIQUES
KW - ANTIQUE MARBLES
KW - CARBONATE ROCK
KW - DIVERSITY
UR - http://www.scopus.com/inward/record.url?scp=84977561726&partnerID=8YFLogxK
U2 - 10.1111/1462-2920.13380
DO - 10.1111/1462-2920.13380
M3 - Article
C2 - 27235544
AN - SCOPUS:84977561726
SN - 1462-2912
JO - Environmental Microbiology
JF - Environmental Microbiology
ER -