A molecular approach to drought-induced reduction in leaf CO2 exchange in drought-resistant Quercus ilex

Jesús Rodríguez-Calcerrada, Ana M. Rodrigues, Pedro Perdiguero, Carla António, Owen K. Atkin, Meng Li, Carmen Collada, Luis Gil

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Drought-induced reduction of leaf gas exchange entails a complex regulation of the plant leaf metabolism. We used a combined molecular and physiological approach to understand leaf photosynthetic and respiratory responses of 2-year-old Quercus ilex seedlings to drought. Mild drought stress resulted in glucose accumulation while net photosynthetic CO2 uptake (Pn) remained unchanged, suggesting a role of glucose in stress signaling and/or osmoregulation. Simple sugars and sugar alcohols increased throughout moderate-to-very severe drought stress conditions, in parallel to a progressive decline in Pn and the quantum efficiency of photosystem II; by contrast, minor changes occurred in respiration rates until drought stress was very severe. At very severe drought stress, 2-oxoglutarate dehydrogenase complex gene expression significantly decreased, and the abundance of most amino acids dramatically increased, especially that of proline and γ-aminobutyric acid (GABA) suggesting enhanced protection against oxidative damage and a reorganization of the tricarboxylic cycle acid cycle via the GABA shunt. Altogether, our results point to Q. ilex drought tolerance being linked to signaling and osmoregulation by hexoses during early stages of drought stress, and enhanced protection against oxidative damage by polyols and amino acids under severe drought stress.

Original languageEnglish
Pages (from-to)394-408
Number of pages15
JournalPhysiologia Plantarum
Volume162
Issue number4
DOIs
Publication statusPublished - 1 Apr 2018

Fingerprint

Quercus
Quercus ilex
Droughts
water stress
drought
leaves
gamma-aminobutyric acid
osmoregulation
Osmoregulation
sugar alcohols
gamma-Aminobutyric Acid
glucose
amino acids
polyols
tricarboxylic acid cycle
hexoses
drought tolerance
Ketoglutarate Dehydrogenase Complex
photosystem II
Sugar Alcohols

Cite this

Rodríguez-Calcerrada, J., Rodrigues, A. M., Perdiguero, P., António, C., Atkin, O. K., Li, M., ... Gil, L. (2018). A molecular approach to drought-induced reduction in leaf CO2 exchange in drought-resistant Quercus ilex. Physiologia Plantarum, 162(4), 394-408. https://doi.org/10.1111/ppl.12649
Rodríguez-Calcerrada, Jesús ; Rodrigues, Ana M. ; Perdiguero, Pedro ; António, Carla ; Atkin, Owen K. ; Li, Meng ; Collada, Carmen ; Gil, Luis. / A molecular approach to drought-induced reduction in leaf CO2 exchange in drought-resistant Quercus ilex. In: Physiologia Plantarum. 2018 ; Vol. 162, No. 4. pp. 394-408.
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abstract = "Drought-induced reduction of leaf gas exchange entails a complex regulation of the plant leaf metabolism. We used a combined molecular and physiological approach to understand leaf photosynthetic and respiratory responses of 2-year-old Quercus ilex seedlings to drought. Mild drought stress resulted in glucose accumulation while net photosynthetic CO2 uptake (Pn) remained unchanged, suggesting a role of glucose in stress signaling and/or osmoregulation. Simple sugars and sugar alcohols increased throughout moderate-to-very severe drought stress conditions, in parallel to a progressive decline in Pn and the quantum efficiency of photosystem II; by contrast, minor changes occurred in respiration rates until drought stress was very severe. At very severe drought stress, 2-oxoglutarate dehydrogenase complex gene expression significantly decreased, and the abundance of most amino acids dramatically increased, especially that of proline and γ-aminobutyric acid (GABA) suggesting enhanced protection against oxidative damage and a reorganization of the tricarboxylic cycle acid cycle via the GABA shunt. Altogether, our results point to Q. ilex drought tolerance being linked to signaling and osmoregulation by hexoses during early stages of drought stress, and enhanced protection against oxidative damage by polyols and amino acids under severe drought stress.",
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Rodríguez-Calcerrada, J, Rodrigues, AM, Perdiguero, P, António, C, Atkin, OK, Li, M, Collada, C & Gil, L 2018, 'A molecular approach to drought-induced reduction in leaf CO2 exchange in drought-resistant Quercus ilex', Physiologia Plantarum, vol. 162, no. 4, pp. 394-408. https://doi.org/10.1111/ppl.12649

A molecular approach to drought-induced reduction in leaf CO2 exchange in drought-resistant Quercus ilex. / Rodríguez-Calcerrada, Jesús; Rodrigues, Ana M.; Perdiguero, Pedro; António, Carla; Atkin, Owen K.; Li, Meng; Collada, Carmen; Gil, Luis.

In: Physiologia Plantarum, Vol. 162, No. 4, 01.04.2018, p. 394-408.

Research output: Contribution to journalArticle

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